Genetic Analysis of the Myotubularin Family of Phosphatases in Caenorhabditis elegans

Yingzi Xue, Hanna Fares, Barth Grant, Zhai Li, Ann M. Rose, Scott G. Clark, Edward Y. Skolnik

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Myotubularins (MTMs) constitute a large family of lipid phosphatases that specifically dephosphorylate phosphatidylinositol (3)P. MTM1 and MTM2 are mutated in X-linked myotubular myopathy and Charcot-Marie-Tooth disease (type 4B), respectively, although the mechanisms whereby MTM dysfunction leads to these diseases is unknown. To gain insight into MTM function, we undertook the study of MTMs in the nematode Caenorhabditis elegans, which possesses representative homologues of the four major subgroups of MTMs identified in mammals. As in mammals, we found that C. elegans MTMs mediate distinct functions. let-512 (vps34) encodes the C. elegans homologue of the yeast and mammalian homologue of the phosphatidylinositol 3-kinase Vps34. We found that reduction of mtm-6 (F53A2.8) function by RNA inhibition rescued the larval lethality of let-512 (vps34) mutants and that the reduction of mtm-1 (Y110A7A.5) activity by RNA inhibition rescued the endocytosis defect of let-512 animals. Together, these observations provide genetic evidence that MTMs negatively regulate phosphatidylinositol (3)P levels. Analysis of MTM expression patterns using transcriptional green fluorescence protein reporters demonstrated that these two MTMs exhibit mostly non-overlapping expression patterns and that MTM-green fluorescence protein fusion proteins are localized to different subcellular locations. These observations suggest that some of the different functions of MTMs might, in part, be a consequence of unique expression and localization patterns. However, our finding that at least three C. elegans MTMs play essential roles in coelomocyte endocytosis, a process that also requires VPS34, indicates that MTMs do not simply turn off VPS34 but unexpectedly also function as positive regulators of biological processes.

Original languageEnglish (US)
Pages (from-to)34380-34386
Number of pages7
JournalJournal of Biological Chemistry
Volume278
Issue number36
DOIs
StatePublished - Sep 5 2003

Fingerprint

Caenorhabditis elegans
Phosphoric Monoester Hydrolases
Mammals
Phosphatidylinositols
Endocytosis
Class III Phosphatidylinositol 3-Kinases
Congenital Structural Myopathies
Fluorescence
RNA
Biological Phenomena
Proteins
Yeast
Animals
Fusion reactions
Yeasts
Lipids
Defects
myotubularin

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Xue, Yingzi ; Fares, Hanna ; Grant, Barth ; Li, Zhai ; Rose, Ann M. ; Clark, Scott G. ; Skolnik, Edward Y. / Genetic Analysis of the Myotubularin Family of Phosphatases in Caenorhabditis elegans. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 36. pp. 34380-34386.
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Genetic Analysis of the Myotubularin Family of Phosphatases in Caenorhabditis elegans. / Xue, Yingzi; Fares, Hanna; Grant, Barth; Li, Zhai; Rose, Ann M.; Clark, Scott G.; Skolnik, Edward Y.

In: Journal of Biological Chemistry, Vol. 278, No. 36, 05.09.2003, p. 34380-34386.

Research output: Contribution to journalArticle

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AU - Fares, Hanna

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