A map of the Bacillus subtilis genome has been constructed from the results of density transfer experiments carried out in strain W23, and from genetic linkage data obtained using DNA-mediated transformation and phage PBS1-mediated transduction in strain 168. Several new linkages have been detected and most of the known linkage groups have been located and oriented relative to the genome replication origin. All of the markers studied except one have been placed in four linkage groups, defined by cotransduction with phage PBS1. The size of the phage PBS1 transducing fragment has been estimated. Our map has been compared with two previously published maps, obtained using the marker-frequency method.
All Science Journal Classification (ASJC) codes
- Structural Biology
- Molecular Biology