Genetic transformation of an entomopathogenic nematode by microinjection

Sarwar Hashmi; Ghazala Hashmi; Randy Gaugler

doi:10.1006/jipa.1995.1103

Genetic transformation of an entomopathogenic nematode by microinjection

Sarwar Hashmi, Ghazala Hashmi, Randy Gaugler

School of Environmental and Biological Sciences, Entomology

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

We report the first successful transformation of an entomopathogenic nematode. Foreign genes were introduced in Heterorhabditis bacteriophora HP88 by microinjection using vectors carrying the Caenorhabditis elegans genes coding for the roller phenotype and 16-kDa heat shock protein (hsp16) gene. A translational fusion made by inserting lacZ in frame into hsp16 was expressed in the body musculature, hypodermis, and pharyngeal muscles. The transcription of the hsp16/lacZ transgenes resulted in the rapid synthesis of detectable levels of β-galactosidase. This research opens new avenues for genetic modification of entomopathogenic nematodes.

Original language	English (US)
Pages (from-to)	293-296
Number of pages	4
Journal	Journal of Invertebrate Pathology
Volume	66
Issue number	3
DOIs	https://doi.org/10.1006/jipa.1995.1103
State	Published - Jan 1 1995

All Science Journal Classification (ASJC) codes

Ecology, Evolution, Behavior and Systematics

Keywords

Caenorhabditis elegans
Entomopathogenic nematodes
Genetic transformation
Heterorhabditis bacteriophora HP88
Microinjection

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10.1006/jipa.1995.1103

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