Genetic transformation of an entomopathogenic nematode by microinjection

Sarwar Hashmi, Ghazala Hashmi, Randy Gaugler

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

We report the first successful transformation of an entomopathogenic nematode. Foreign genes were introduced in Heterorhabditis bacteriophora HP88 by microinjection using vectors carrying the Caenorhabditis elegans genes coding for the roller phenotype and 16-kDa heat shock protein (hsp16) gene. A translational fusion made by inserting lacZ in frame into hsp16 was expressed in the body musculature, hypodermis, and pharyngeal muscles. The transcription of the hsp16/lacZ transgenes resulted in the rapid synthesis of detectable levels of β-galactosidase. This research opens new avenues for genetic modification of entomopathogenic nematodes.

Original languageEnglish (US)
Pages (from-to)293-296
Number of pages4
JournalJournal of Invertebrate Pathology
Volume66
Issue number3
DOIs
StatePublished - Jan 1 1995

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics

Keywords

  • Caenorhabditis elegans
  • Entomopathogenic nematodes
  • Genetic transformation
  • Heterorhabditis bacteriophora HP88
  • Microinjection

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