Genetic transformation of nematodes using arrays of micromechanical piercing structures

S. Hashmi, P. Ling, G. Hashmi, M. Reed, R. Gaugler, W. Trimmer

Research output: Contribution to journalArticlepeer-review

88 Scopus citations

Abstract

Foreign genes were expressed in the nematode Heterorhabditis bacteriophora using a new micro-mechanical device to inject DNA-coated microprobe through the nematode cuticle. After introduction of a plasmid in which a Caenorhabditis elegans 16-kDa heat shock promoter was fused to Escherichia coli, a β-galactosidase gene was expressed in the progeny of the injected worms. The tip of the microprobe penetrated through the nematode cuticle without causing injury to the nematode, and about 8% of the total progeny tested expressed the foreign gene. We describe and validate a new and efficient genetic transformation system for nematodes. The method is quick and easy to use.

Original languageEnglish (US)
Pages (from-to)766-770
Number of pages5
JournalBioTechniques
Volume19
Issue number5
StatePublished - 1995

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry, Genetics and Molecular Biology(all)

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