TY - JOUR
T1 - Genomic analysis identifies association of Fusobacterium with colorectal carcinoma
AU - Kostic, Aleksandar D.
AU - Gevers, Dirk
AU - Pedamallu, Chandra Sekhar
AU - Michaud, Monia
AU - Duke, Fujiko
AU - Earl, Ashlee M.
AU - Ojesina, Akinyemi I.
AU - Jung, Joonil
AU - Bass, Adam J.
AU - Tabernero, Josep
AU - Baselga, José
AU - Liu, Chen
AU - Shivdasani, Ramesh A.
AU - Ogino, Shuji
AU - Birren, Bruce W.
AU - Huttenhower, Curtis
AU - Garrett, Wendy S.
AU - Meyerson, Matthew
PY - 2012/2
Y1 - 2012/2
N2 - The tumor microenvironment of colorectal carcinoma is a complex community of genomically altered cancer cells, nonneoplastic cells, and a diverse collection of microorganisms. Each of these components may contribute to carcinogenesis; however, the role of the microbiota is the least well understood. We have characterized the composition of the microbiota in colorectal carcinoma using whole genome sequences from nine tumor/normal pairs. Fusobacterium sequences were enriched in carcinomas, confirmed by quantitative PCR and 16S rDNA sequence analysis of 95 carcinoma/normal DNA pairs, while the Bacteroidetes and Firmicutes phyla were depleted in tumors. Fusobacteria were also visualized within colorectal tumors using FISH. These findings reveal alterations in the colorectal cancer microbiota; however, the precise role of Fusobacteria in colorectal carcinoma pathogenesis requires further investigation.
AB - The tumor microenvironment of colorectal carcinoma is a complex community of genomically altered cancer cells, nonneoplastic cells, and a diverse collection of microorganisms. Each of these components may contribute to carcinogenesis; however, the role of the microbiota is the least well understood. We have characterized the composition of the microbiota in colorectal carcinoma using whole genome sequences from nine tumor/normal pairs. Fusobacterium sequences were enriched in carcinomas, confirmed by quantitative PCR and 16S rDNA sequence analysis of 95 carcinoma/normal DNA pairs, while the Bacteroidetes and Firmicutes phyla were depleted in tumors. Fusobacteria were also visualized within colorectal tumors using FISH. These findings reveal alterations in the colorectal cancer microbiota; however, the precise role of Fusobacteria in colorectal carcinoma pathogenesis requires further investigation.
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U2 - 10.1101/gr.126573.111
DO - 10.1101/gr.126573.111
M3 - Article
C2 - 22009990
AN - SCOPUS:84863022950
SN - 1088-9051
VL - 22
SP - 292
EP - 298
JO - Genome research
JF - Genome research
IS - 2
ER -