Genomic analysis identifies association of Fusobacterium with colorectal carcinoma

Aleksandar D. Kostic; Dirk Gevers; Chandra Sekhar Pedamallu; Monia Michaud; Fujiko Duke; Ashlee M. Earl; Akinyemi I. Ojesina; Joonil Jung; Adam J. Bass; Josep Tabernero; José Baselga; Chen Liu; Ramesh A. Shivdasani; Shuji Ogino; Bruce W. Birren; Curtis Huttenhower; Wendy S. Garrett; Matthew Meyerson

doi:10.1101/gr.126573.111

Genomic analysis identifies association of Fusobacterium with colorectal carcinoma

Aleksandar D. Kostic, Dirk Gevers, Chandra Sekhar Pedamallu, Monia Michaud, Fujiko Duke, Ashlee M. Earl, Akinyemi I. Ojesina, Joonil Jung, Adam J. Bass, Josep Tabernero, José Baselga, Chen Liu, Ramesh A. Shivdasani, Shuji Ogino, Bruce W. Birren, Curtis Huttenhower, Wendy S. Garrett, Matthew Meyerson

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1145 Scopus citations

Abstract

The tumor microenvironment of colorectal carcinoma is a complex community of genomically altered cancer cells, nonneoplastic cells, and a diverse collection of microorganisms. Each of these components may contribute to carcinogenesis; however, the role of the microbiota is the least well understood. We have characterized the composition of the microbiota in colorectal carcinoma using whole genome sequences from nine tumor/normal pairs. Fusobacterium sequences were enriched in carcinomas, confirmed by quantitative PCR and 16S rDNA sequence analysis of 95 carcinoma/normal DNA pairs, while the Bacteroidetes and Firmicutes phyla were depleted in tumors. Fusobacteria were also visualized within colorectal tumors using FISH. These findings reveal alterations in the colorectal cancer microbiota; however, the precise role of Fusobacteria in colorectal carcinoma pathogenesis requires further investigation.

Original language	English (US)
Pages (from-to)	292-298
Number of pages	7
Journal	Genome research
Volume	22
Issue number	2
DOIs	https://doi.org/10.1101/gr.126573.111
State	Published - Feb 2012
Externally published	Yes

All Science Journal Classification (ASJC) codes

Genetics
Genetics(clinical)

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Kostic, A. D., Gevers, D., Pedamallu, C. S., Michaud, M., Duke, F., Earl, A. M., Ojesina, A. I., Jung, J., Bass, A. J., Tabernero, J., Baselga, J., Liu, C., Shivdasani, R. A., Ogino, S., Birren, B. W., Huttenhower, C., Garrett, W. S., & Meyerson, M. (2012). Genomic analysis identifies association of Fusobacterium with colorectal carcinoma. Genome research, 22(2), 292-298. https://doi.org/10.1101/gr.126573.111

@article{edf29a61610b4b72a7ed1a1be0094f73,

title = "Genomic analysis identifies association of Fusobacterium with colorectal carcinoma",

abstract = "The tumor microenvironment of colorectal carcinoma is a complex community of genomically altered cancer cells, nonneoplastic cells, and a diverse collection of microorganisms. Each of these components may contribute to carcinogenesis; however, the role of the microbiota is the least well understood. We have characterized the composition of the microbiota in colorectal carcinoma using whole genome sequences from nine tumor/normal pairs. Fusobacterium sequences were enriched in carcinomas, confirmed by quantitative PCR and 16S rDNA sequence analysis of 95 carcinoma/normal DNA pairs, while the Bacteroidetes and Firmicutes phyla were depleted in tumors. Fusobacteria were also visualized within colorectal tumors using FISH. These findings reveal alterations in the colorectal cancer microbiota; however, the precise role of Fusobacteria in colorectal carcinoma pathogenesis requires further investigation.",

author = "Kostic, {Aleksandar D.} and Dirk Gevers and Pedamallu, {Chandra Sekhar} and Monia Michaud and Fujiko Duke and Earl, {Ashlee M.} and Ojesina, {Akinyemi I.} and Joonil Jung and Bass, {Adam J.} and Josep Tabernero and Jos{\'e} Baselga and Chen Liu and Shivdasani, {Ramesh A.} and Shuji Ogino and Birren, {Bruce W.} and Curtis Huttenhower and Garrett, {Wendy S.} and Matthew Meyerson",

year = "2012",

month = feb,

doi = "10.1101/gr.126573.111",

language = "English (US)",

volume = "22",

pages = "292--298",

journal = "Genome Research",

issn = "1088-9051",

publisher = "Cold Spring Harbor Laboratory Press",

number = "2",

}

Kostic, AD, Gevers, D, Pedamallu, CS, Michaud, M, Duke, F, Earl, AM, Ojesina, AI, Jung, J, Bass, AJ, Tabernero, J, Baselga, J, Liu, C, Shivdasani, RA, Ogino, S, Birren, BW, Huttenhower, C, Garrett, WS & Meyerson, M 2012, 'Genomic analysis identifies association of Fusobacterium with colorectal carcinoma', Genome research, vol. 22, no. 2, pp. 292-298. https://doi.org/10.1101/gr.126573.111

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AU - Kostic, Aleksandar D.

AU - Gevers, Dirk

AU - Pedamallu, Chandra Sekhar

AU - Michaud, Monia

AU - Duke, Fujiko

AU - Earl, Ashlee M.

AU - Ojesina, Akinyemi I.

AU - Jung, Joonil

AU - Bass, Adam J.

AU - Tabernero, Josep

AU - Baselga, José

AU - Liu, Chen

AU - Shivdasani, Ramesh A.

AU - Ogino, Shuji

AU - Birren, Bruce W.

AU - Huttenhower, Curtis

AU - Garrett, Wendy S.

AU - Meyerson, Matthew

PY - 2012/2

Y1 - 2012/2

N2 - The tumor microenvironment of colorectal carcinoma is a complex community of genomically altered cancer cells, nonneoplastic cells, and a diverse collection of microorganisms. Each of these components may contribute to carcinogenesis; however, the role of the microbiota is the least well understood. We have characterized the composition of the microbiota in colorectal carcinoma using whole genome sequences from nine tumor/normal pairs. Fusobacterium sequences were enriched in carcinomas, confirmed by quantitative PCR and 16S rDNA sequence analysis of 95 carcinoma/normal DNA pairs, while the Bacteroidetes and Firmicutes phyla were depleted in tumors. Fusobacteria were also visualized within colorectal tumors using FISH. These findings reveal alterations in the colorectal cancer microbiota; however, the precise role of Fusobacteria in colorectal carcinoma pathogenesis requires further investigation.

AB - The tumor microenvironment of colorectal carcinoma is a complex community of genomically altered cancer cells, nonneoplastic cells, and a diverse collection of microorganisms. Each of these components may contribute to carcinogenesis; however, the role of the microbiota is the least well understood. We have characterized the composition of the microbiota in colorectal carcinoma using whole genome sequences from nine tumor/normal pairs. Fusobacterium sequences were enriched in carcinomas, confirmed by quantitative PCR and 16S rDNA sequence analysis of 95 carcinoma/normal DNA pairs, while the Bacteroidetes and Firmicutes phyla were depleted in tumors. Fusobacteria were also visualized within colorectal tumors using FISH. These findings reveal alterations in the colorectal cancer microbiota; however, the precise role of Fusobacteria in colorectal carcinoma pathogenesis requires further investigation.

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Genomic analysis identifies association of Fusobacterium with colorectal carcinoma

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