Genomic and Proteomic Analysis of phiEco32, a Novel Escherichia coli Bacteriophage

Dhruti Savalia, Lars F. Westblade, Manisha Goel, Laurence Florens, Priscilla Kemp, Natalja Akulenko, Olga Pavlova, Julio C. Padovan, Brian T. Chait, Michael P. Washburn, Hans W. Ackermann, Arcady Mushegian, Tarasii Gabisonia, Ian Molineux, Konstantin Severinov

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

A novel bacteriophage infecting Escherichia coli was isolated during a large-scale screen for bacteriophages that may be used for therapy of mastitis in cattle. The 77,554-bp genome of the bacteriophage, named phiEco32, was sequenced and annotated, and its virions were characterized by electron microscopy and proteomics. Two phiEco32-encoded proteins that interact with host RNA polymerase were identified. One of them is an ECF family σ factor that may be responsible for transcription of some viral genes. Another RNA polymerase-binding protein is a novel transcription inhibitor whose mechanism of action remains to be defined.

Original languageEnglish (US)
Pages (from-to)774-789
Number of pages16
JournalJournal of molecular biology
Volume377
Issue number3
DOIs
StatePublished - Mar 28 2008

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

Keywords

  • E. coli
  • MudPIT
  • Podoviridae
  • bacteriophage
  • genome

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