Genomic hybrid capture assay to detect Borrelia burgdorferi: an application to diagnose neuroborreliosis in horses

Thomas J. Divers, Emmanuel F. Mongodin, Christopher B. Miller, Rodney L. Belgrave, Rachel B. Gardner, Claire M. Fraser, Steven E. Schutzer

Research output: Contribution to journalArticlepeer-review

Abstract

Antemortem diagnosis of neuroborreliosis in horses has been hindered by both the low sensitivity of PCR testing for Borrelia burgdorferi in CSF and the low specificity of serum:CSF ELISA ratios used to determine intrathecal antibody production against the bacterium. PCR testing of the CSF of an adult horse with acute neurologic disease for the B. burgdorferi flagellin gene was negative. However, we enriched B. burgdorferi DNA through nucleic acid hybrid capture, followed by next-generation sequencing, and identified B. burgdorferi in the CSF of the horse, confirming a diagnosis of neuroborreliosis.

Original languageEnglish (US)
Pages (from-to)909-912
Number of pages4
JournalJournal of Veterinary Diagnostic Investigation
Volume34
Issue number5
DOIs
StatePublished - Sep 2022

All Science Journal Classification (ASJC) codes

  • General Veterinary

Keywords

  • Lyme disease
  • genomic hybrid capture assay
  • horses
  • neuroborreliosis

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