Abstract
Antemortem diagnosis of neuroborreliosis in horses has been hindered by both the low sensitivity of PCR testing for Borrelia burgdorferi in CSF and the low specificity of serum:CSF ELISA ratios used to determine intrathecal antibody production against the bacterium. PCR testing of the CSF of an adult horse with acute neurologic disease for the B. burgdorferi flagellin gene was negative. However, we enriched B. burgdorferi DNA through nucleic acid hybrid capture, followed by next-generation sequencing, and identified B. burgdorferi in the CSF of the horse, confirming a diagnosis of neuroborreliosis.
Original language | English (US) |
---|---|
Pages (from-to) | 909-912 |
Number of pages | 4 |
Journal | Journal of Veterinary Diagnostic Investigation |
Volume | 34 |
Issue number | 5 |
DOIs | |
State | Published - Sep 2022 |
All Science Journal Classification (ASJC) codes
- General Veterinary
Keywords
- Lyme disease
- genomic hybrid capture assay
- horses
- neuroborreliosis