GLUT-5 expression in neonatal rats: Crypt-villus location and age-dependent regulation

L. Jiang, E. S. David, N. Espina, R. P. Ferraris

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

The rat fructose transporter normally appears after completion of weaning but can be precociously induced by early feeding of a high-fructose diet. In this study, the crypt-villus site, the metabolic nature of the signal, and the age dependence of induction were determined. In weaning rats fed high-glucose pellets, GLUT-5 mRNA expression was modest, localized mainly in the upper three-fourths of the villus, and there was little expression in the villus base. When fed high-fructose pellets, GLUT-5 mRNA expression was two to three times greater in all regions except the villus base. Intestinal perfusion in vivo of a nonmetabolizable fructose analog, 3-O-methylfructose, tended to increase fructose uptake rate and moderately increased GLUT-5 mRNA abundance but had no effect on glucose uptake rates and SGLT1 mRNA abundance. Gavage feeding of high-fructose, but not high-glucose, solutions enhanced fructose uptake only in pups ≥14 days, suggesting that GLUT-5 regulation is markedly age dependent. Fructose or its metabolites upregulate GLUT-5 expression in all enterocytes, except those in the crypt and villus base and in pups <14 days old.

Original languageEnglish (US)
Pages (from-to)G666-G674
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume281
Issue number3 44-3
DOIs
StatePublished - 2001

All Science Journal Classification (ASJC) codes

  • Physiology
  • Hepatology
  • Gastroenterology
  • Physiology (medical)

Keywords

  • Membranes
  • Metabolism
  • Small intestine
  • Sugars
  • Transport

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