Glycoconjugate profiling of primary melanoma and its sentinel node and distant metastases: Implications for diagnosis and pathophysiology of metastases

Anka Thies, Anke Berlin, Georg Brunner, Hans Joachim Schulze, Ingrid Moll, Uwe Pfüller, Christoph Wagener, Melitta Schachner, Peter Altevogt, Udo Schumacher

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Aiming at more precise detection of melanoma cells in sentinel lymph nodes and better understanding of the mechanisms underlying metastatic spread, expression of L1, CEACAM1, and binding of the lectins HPA, ML-I and PNA, was assessed in benign nevi (n = 12), primary melanomas (PTs: n = 67), their corresponding sentinel lymph nodes (SLNs: n = 40), and distant metastases (DMs: n = 35). Sensitivity and specificity of CEACAM1 (95-97%; 66%) and L1 (90-93%; 100%) exceeded that of the standard markers MelanA, S100, and HMB45 in single marker use. Lectin binding was found in PTs and DMs (HPA: 69% and 77%; ML-I: 82% and 77%, respectively), but rarely in SLNMs (HPA: 20%, ML-I: 20%, PNA: 5%, respectively). The highly specific and sensitive L1-11A against L1 and 4D1/C2 against CEACAM1 antibodies are a worthy completion to standard antibody panels for diagnosis of melanoma cells. Both CAMs seem to be functionally involved in lymphatic and haematogenous spread, and are thus promising target molecules for immunotoxins.

Original languageEnglish (US)
Pages (from-to)68-80
Number of pages13
JournalCancer Letters
Volume248
Issue number1
DOIs
StatePublished - Apr 8 2007
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Keywords

  • CEACAM1
  • L1
  • Lectins
  • Malignant melanoma
  • Metastasis
  • Sentinel lymph node

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