Glycosaminoglycan patterns in gingival proteoglycans of rat with age

M. Weinstein, Y. H. Liau, A. Slomiany, B. L. Slomiany

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Among the potential biochemical indices that are closely associated with craniofacial development are the proteoglycans. Gingival segments from the palate of 4-,6-,8-,12- and 18-week-old rats were incubated for 4 h in medium containing [3H]-glucosamine and [35S]-Na2SO4, and subjected to proteoglycan isolation and glycosaminoglycan analysis. Two distinct proteoglycan fractions differing in the degree of sulphation were obtained by ion-exchange chromatography. The incorporation of both labels in the undersulphated fraction increased with age; there was a pronounced decrease with age in the sulphated proteoglycan fraction. The undersulphated proteoglycans showed an age-dependent decrease in hyaluronic acid, and increase in dermatan sulphate and chondroitin 4- and 6-sulphates. Gel filtration of the sulphated proteoglycan fraction yielded high and low molecular-weight proteoglycans, the glycosaminoglycans of which were particularly rich (61-76%) in dermatan sulphate. Smaller quantities of chondroitin 4- and 6-sulphates, and heparan sulphate were also present. All glycosaminoglycans showed a decrease in content with age. The findings suggest a possible correlation between gingival proteoglycan/glycosaminoglycan patterns and development.

Original languageEnglish (US)
Pages (from-to)323-330
Number of pages8
JournalArchives of Oral Biology
Volume37
Issue number5
DOIs
StatePublished - May 1992

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology
  • Dentistry(all)
  • Cell Biology

Keywords

  • developmental changes
  • gingiva
  • glycosaminoglycan
  • patterns
  • proteoglycan
  • rat

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