Glycosulfatase activity of porphyromonas gingivalis. A bacterium associated with periodontal disease

B. L. Slomiany, V. L.N. Murty, J. Piotrowski, Y. H. Liau, A. Slomiany

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

Porphyromonas gingivalis, a bacterium implicated in the pathogenesis of periodontal disease, was found to elaborate an extracellular glycosulfatase enzyme. Upon purification by low temperature acetone fractionation, an active enzyme at 60% acetone was obtained which on SDS-PAGE gave a protein band of 37kDa. The glycosulfatase effectively caused desulfation of galactosyl- and lactosylceramide sulfates (pH 5.0) which contain the sulfate ester groups at C-3 of galactose, a well as proteoglycans (pH 5.7-6.2) of gingival tissue which are rich in N-acetylgalactosamine-4-sulfate, but not the sulfated salivary mucin with the sulfate groups at C-6 of galactose and C-6 of N-acetylglucosamine. The results demonstrate for the first time that P. gingivalis displays glycosulfatase activity and that the disruptive action of this enzyme may be a major factor in the etiology of periodontal disease.

Original languageEnglish (US)
Pages (from-to)973-980
Number of pages8
JournalBiochemistry and Molecular Biology International
Volume29
Issue number5
StatePublished - 1993

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Genetics

Fingerprint

Dive into the research topics of 'Glycosulfatase activity of porphyromonas gingivalis. A bacterium associated with periodontal disease'. Together they form a unique fingerprint.

Cite this