GM1 ganglioside modulates prostaglandin E1 stimulated adenylyl cyclase in neuro-2a cells

Gusheng Wu, Zi Hua Lu, Robert W. Ledeen

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

This study demonstrates modulation by GM1 ganglioside of prostaglandin E1 (PGE1)-induced cAMP formation in Neuro-2a neuroblastoma cells. Pretreatment of the cells with neuraminidase, an enzyme that increases cell surface GM1, resulted in significant elevation of PGE1-induced cAMP formation, as did preincubation of the cells with nmolar concentrations of GM1. Pretreatment with brain ganglioside mixture lacking GM1 had no effect. Cholera toxin B subunit, a specific GM1-binding ligand, inhibited adenylyl cyclase. When the concentration of exogenous GM1 in which the cells were preincubated was increased from nmolar to μmolar levels there was a dose-responsive fall off in cAMP elevation, attributed to progressive inhibition of adenylyl cyclase by increasing GM1. These results are interpreted as indicating modulation of this PGE1 receptor in Neuro-2a cells by plasma membrane-localized GM1 in a structure-specific manner.

Original languageEnglish (US)
Pages (from-to)235-239
Number of pages5
JournalGlycoconjugate Journal
Volume13
Issue number2
DOIs
StatePublished - 1996

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Keywords

  • Adenylyl cyclase
  • Cholera toxin B subunit
  • Cyclase AMP
  • GM1 as receptor modulator
  • GM1 ganglioside
  • Gangliosides
  • Neuraminidase
  • Prostaglandin receptor

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