Green fluorescent protein as a marker for gene expression

Martin Chalfie, Yuan Tu, Ghia Euskirchen, William W. Ward, Douglas C. Prasher

Research output: Contribution to journalArticlepeer-review

5226 Scopus citations

Abstract

A complementary DNA for the Aequorea victoria green fluorescent protein (GFP) produces a fluorescent product when expressed in prokaryotic (Escherichia coli) or eukaryotic (Caenorhabditis elegans) cells. Because exogenous substrates and cofactors are not required for this fluorescence, GFP expression can be used to monitor gene expression and protein localization in living organisms.

Original languageEnglish (US)
Pages (from-to)802-805
Number of pages4
JournalScience
Volume263
Issue number5148
DOIs
StatePublished - 1994

All Science Journal Classification (ASJC) codes

  • General

Fingerprint Dive into the research topics of 'Green fluorescent protein as a marker for gene expression'. Together they form a unique fingerprint.

Cite this