TY - JOUR
T1 - Green Tea Catechins Effectively Altered Hepatic Fibrogenesis in Rats by Inhibiting ERK and Smad1/2 Phosphorylation
AU - Wang, Liwen
AU - Yang, Guliang
AU - Yuan, Li
AU - Yang, Yiwen
AU - Zhao, Hui
AU - Ho, Chi Tang
AU - Li, Shiming
N1 - Funding Information:
*E-mail: 469436773@qq.com. *Telephone: 1-973-919-3702. Fax: 1-732-932-6776. E-mail: shiming@rutgers.edu. ORCID Liwen Wang: 0000-0001-7135-3418 Hui Zhao: 0000-0002-7517-5054 Chi-Tang Ho: 0000-0001-8273-2085 Shiming Li: 0000-0002-6167-0660 Funding This study was supported by the National Natural Science Foundation of China (Grants 81172837 and 31571832), the Tianjin Innovative Research Team Grant (TD-12-5049), the Tianjin Innovative Research Team Grant (TD-13-5087), and the Open Grant from Tianjin Key Laboratory of Food Biotechnology (TJCU-KLFB-18103). Notes The authors declare no competing financial interest.
Publisher Copyright:
© 2018 American Chemical Society.
PY - 2019/5/15
Y1 - 2019/5/15
N2 - Polyphenols derived from green tea have been reported to have a wide range of profound functions. Tea catechins, including epicatechin, epigallocatechin (EGC), epicatechin-3-O-gallate (ECG), and epigallocatechin-3-O-gallate (EGCG), are considered as the major bioactive polyphenols in tea. The present study was designed to elucidate the potential antifibrogenic role of three abundant tea catechins (ECG, EGC, and EGCG) in a CCl 4 -induced fibrotic rat and their underlying molecular mechanisms. Tea catechins, especially groups of ECG, EGC, and EGCG, effectively induced several beneficial alterations of liver injury markers, oxidative status, and liver histology. Furthermore, catechins ameliorated liver fibrosis, as evidenced by the reduced expression of desmin, α-smooth muscle actin, transforming growth factor β (TGF-β), and downstream ERK1/2 and Smad1/2 phosphorylation. The most significant inhibitory effect on those proteins was observed in ECG (300 mg/kg) and EGCG (300 mg/kg) groups. In addition, catechins conferred their protective role by downregulating the proinflammation cytokines TGF-β, tumor necrosis factor α, and interleukin 17. It is postulated that tea catechins, particularly ECG and EGCG, are potential therapeutic candidates in antifibrotic therapy.
AB - Polyphenols derived from green tea have been reported to have a wide range of profound functions. Tea catechins, including epicatechin, epigallocatechin (EGC), epicatechin-3-O-gallate (ECG), and epigallocatechin-3-O-gallate (EGCG), are considered as the major bioactive polyphenols in tea. The present study was designed to elucidate the potential antifibrogenic role of three abundant tea catechins (ECG, EGC, and EGCG) in a CCl 4 -induced fibrotic rat and their underlying molecular mechanisms. Tea catechins, especially groups of ECG, EGC, and EGCG, effectively induced several beneficial alterations of liver injury markers, oxidative status, and liver histology. Furthermore, catechins ameliorated liver fibrosis, as evidenced by the reduced expression of desmin, α-smooth muscle actin, transforming growth factor β (TGF-β), and downstream ERK1/2 and Smad1/2 phosphorylation. The most significant inhibitory effect on those proteins was observed in ECG (300 mg/kg) and EGCG (300 mg/kg) groups. In addition, catechins conferred their protective role by downregulating the proinflammation cytokines TGF-β, tumor necrosis factor α, and interleukin 17. It is postulated that tea catechins, particularly ECG and EGCG, are potential therapeutic candidates in antifibrotic therapy.
KW - ERK inhibition
KW - antifibrosis
KW - catechins
KW - hepatic fibrogenesis
KW - rats
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U2 - 10.1021/acs.jafc.8b05179
DO - 10.1021/acs.jafc.8b05179
M3 - Article
C2 - 30424599
AN - SCOPUS:85057867823
SN - 0021-8561
VL - 67
SP - 5437
EP - 5445
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
IS - 19
ER -