@article{aa6668e7d76a40bab7873f1d9457f1d6,
title = "Green Tea Polyphenols Modify the Gut Microbiome in db/db Mice as Co-Abundance Groups Correlating with the Blood Glucose Lowering Effect",
abstract = "Scope: The effects of green tea polyphenols, Polyphenon E (PPE), and black tea polyphenols, theaflavins (TFs), on gut microbiota and development of diabetes in db/db mice are investigated and compared. Methods and results: Supplementation of PPE (0.1%) in the diet of female db/db mice for 7 weeks decreases fasting blood glucose levels and mesenteric fat while increasing the serum level of insulin, possibly through protection against β-cell damage. However, TFs are less or not effective. Microbiome analysis through 16S rRNA gene sequencing shows that PPE and TFs treatments significantly alter the bacterial community structure in the cecum and colon, but not in the ileum. The key bacterial phylotypes responding to the treatments are then clustered into 11 co-abundance groups (CAGs). CAGs 6 and 7, significantly increased by PPE but not by TFs, are negatively associated with blood glucose levels. The operational taxonomic units in these CAGs are from two different phyla, Firmicutes and Bacteroidetes. CAG 10, decreased by PPE and TFs, is positively associated with blood glucose levels. Conclusion: Gut microbiota respond to tea polyphenol treatments as CAGs instead of taxa. Some of the CAGs associated with the blood glucose lowering effect are enriched by PPE, but not TFs.",
keywords = "db/db mice, diabetes, intestinal microbiota, metabolism, tea polyphenols",
author = "Tingting Chen and Liu, {Anna B.} and Shili Sun and Ajami, {Nadim J.} and Ross, {Matthew C.} and Hong Wang and Le Zhang and Kenneth Reuhl and Koichi Kobayashi and Onishi, {Janet C.} and Liping Zhao and Yang, {Chung S.}",
note = "Funding Information: T.C., A.B.L, and S.S. contributed equally to this study. C.S.Y. and A.B.L. designed this study. J.C.O. and K.K. contributed ideas for the microbiota study. A.B.L., S.S., L.Z., and H.W. conducted animal experiments and biochemical analysis, K.R. performed histological analysis. N.J.A. and M.C.R. conducted 16s rRNA sequencing analysis. T.C. and L.Z. conducted microbiome informatics analysis; C.S.Y., T.C., J.C.O., and L.Z. prepared the manuscript. The authors thank Dr. Y. Hara (Mitsui Norin Co.) and Dr. Y. Gong (Hunan Agricultural University) for providing the PPE and TFs for the study; Rutgers Discovery Informatics Institute (RDI2) and Advanced Computing and Data Cyberinfrastructure for providing computing system; Mr. Jing Wang and Dr. Guojun Wu of Jiao Tong University for technical support on the bioinformatics data analysis; and Ms. Vi Dan for assistance in the preparation of this manuscript. This work was supported by a pilot project award from CEED at Rutgers University (NIH grant ES005022), and NIH grant CA133024 (to C.S.Y.), and NIH grant ES023512 to the Center for Precision Environmental Health at the Baylor College of Medicine (N.J.A. and M.C.R.). The raw Illumina sequence data has been uploaded to the sequence read archive at NCBI under Bioproject accession PRJNA480760. Funding Information: T.C., A.B.L, and S.S. contributed equally to this study. C.S.Y. and A.B.L. designed this study. J.C.O. and K.K. contributed ideas for the microbiota study. A.B.L., S.S., L.Z., and H.W. conducted animal experiments and biochemical analysis, K.R. performed histological analysis. N.J.A. and M.C.R. conducted 16s rRNA sequencing analysis. T.C. and L.Z. conducted mi-crobiome informatics analysis; C.S.Y., T.C., J.C.O., and L.Z. prepared the manuscript. The authors thank Dr. Y. Hara (Mitsui Norin Co.) and Dr. Y. Gong (Hunan Agricultural University) for providing the PPE and TFs for the study; Rutgers Discovery Informatics Institute (RDI2) and Advanced Computing and Data Cyberinfrastructure for providing computing system; Mr. Jing Wang and Dr. Guojun Wu of Jiao Tong University for technical support on the bioinformatics data analysis; and Ms. Vi Dan for assistance in the preparation of this manuscript. This work was supported by a pilot project award from CEED at Rutgers University (NIH grant ES005022), and NIH grant CA133024 (to C.S.Y.), and NIH grant ES023512 to the Center for Precision Environmental Health at the Baylor College of Medicine (N.J.A. and M.C.R.). The raw Illumina sequence data has been uploaded to the sequence read archive at NCBI under Bioproject accession PRJNA480760. Publisher Copyright: {\textcopyright} 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim",
year = "2019",
month = apr,
doi = "10.1002/mnfr.201801064",
language = "English (US)",
volume = "63",
journal = "Die Nahrung",
issn = "1613-4125",
publisher = "Wiley-VCH Verlag",
number = "8",
}