Guided axon growth by gradients of adhesion in collagen gels

Harini G. Sundararaghavan, Gary A. Monteiro, David I. Shreiber

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

During development, neurites are directed by gradients of attractive and repulsive soluble (chemotactic) cues and substrate-bound adhesive (haptotactic) cues. Many of these cues have been extensively researched in vitro, and incorporated into strategies for nerve and spinal cord regeneration, primarily to improve the regenerative environment. To enhance and direct growth, we have developed a system to create 1D gradients of adhesion through a 3D collagen gel using microfluidics. We test our system using collagen grafted with bioactive peptide sequences, IKVAV and YIGSR, from laminin - an extra-cellular matrix (ECM) protein known to strongly influence neurite outgrowth. Gradients are established from sim;0.37mg peptide/mg collagen - 0, and ∼0.18 mg peptide/mg collagen - 0 of each peptide and tested using chick dorsal root ganglia (DRG). Neurite growth is evaluated 5 days after gradient formation. Neurites show increased growth in the gradient system when compared to control and biased growth up the gradient of peptides. Growth in Y1GSR-grafted collagen increased with steeper gradients, whereas growth in 1KVAV-grafted collagen decreased with steeper gradients. These results demonstrate that neurite growth can be enhanced and directed by controlled, immobilized, haptotactic gradients through 3D scaffolds, and suggest that including these gradients in regenerative therapies may accelerate nerve and spinal cord regeneration.

Original languageEnglish (US)
Title of host publication2008 Proceedings of ASME International Mechanical Engineering Congress and Exposition, IMECE 2008
Pages31-37
Number of pages7
DOIs
StatePublished - 2009
Event2008 ASME International Mechanical Engineering Congress and Exposition, IMECE 2008 - Boston, MA, United States
Duration: Oct 31 2008Nov 6 2008

Publication series

NameASME International Mechanical Engineering Congress and Exposition, Proceedings
Volume2

Other

Other2008 ASME International Mechanical Engineering Congress and Exposition, IMECE 2008
Country/TerritoryUnited States
CityBoston, MA
Period10/31/0811/6/08

All Science Journal Classification (ASJC) codes

  • Mechanical Engineering

Fingerprint

Dive into the research topics of 'Guided axon growth by gradients of adhesion in collagen gels'. Together they form a unique fingerprint.

Cite this