Helicase promotes replication re-initiation from an RNA transcript

Bo Sun, Anupam Singh, Shemaila Sultana, James T. Inman, Smita Patel, Michelle D. Wang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

To ensure accurate DNA replication, a replisome must effectively overcome numerous obstacles on its DNA substrate. After encountering an obstacle, a progressing replisome often aborts DNA synthesis but continues to unwind. However, little is known about how DNA synthesis is resumed downstream of an obstacle. Here, we examine the consequences of a non-replicating replisome collision with a co-directional RNA polymerase (RNAP). Using single-molecule and ensemble methods, we find that T7 helicase interacts strongly with a non-replicating T7 DNA polymerase (DNAP) at a replication fork. As the helicase advances, the associated DNAP also moves forward. The presence of the DNAP increases both helicase's processivity and unwinding rate. We show that such a DNAP, together with its helicase, is indeed able to actively disrupt a stalled transcription elongation complex, and then initiates replication using the RNA transcript as a primer. These observations exhibit T7 helicase's novel role in replication re-initiation.

Original languageEnglish (US)
Article number2306
JournalNature communications
Volume9
Issue number1
DOIs
StatePublished - Dec 1 2018

Fingerprint

DNA-Directed DNA Polymerase
deoxyribonucleic acid
RNA
DNA
DNA-Directed RNA Polymerases
Transcription
DNA Replication
Elongation
primers
forks
Molecules
Substrates
synthesis
elongation
collisions
molecules

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Physics and Astronomy(all)

Cite this

Sun, Bo ; Singh, Anupam ; Sultana, Shemaila ; Inman, James T. ; Patel, Smita ; Wang, Michelle D. / Helicase promotes replication re-initiation from an RNA transcript. In: Nature communications. 2018 ; Vol. 9, No. 1.
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Helicase promotes replication re-initiation from an RNA transcript. / Sun, Bo; Singh, Anupam; Sultana, Shemaila; Inman, James T.; Patel, Smita; Wang, Michelle D.

In: Nature communications, Vol. 9, No. 1, 2306, 01.12.2018.

Research output: Contribution to journalArticle

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AU - Sun, Bo

AU - Singh, Anupam

AU - Sultana, Shemaila

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AU - Wang, Michelle D.

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