Helicobacter pylori lipopolysaccharide effect on the synthesis and secretion of gastric sulfomucin

Y. H. Liau, R. A. Lopez, A. Slomiany, B. L. Slomiany

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22 Scopus citations

Abstract

The effect of H. pylori lipopolysaccharide on the synthesis and secretion of sulfated mucin in gastric mucosa was investigated using mucosal segments incubated in the presence of [3H]proline, [3H]glucosamine and [35S]Na2SO4. The lipopolysaccharide, while showing no discernible effect on the apomucin synthesis was found to inhibit the process of mucin glycosylation and sulfation, which at 100μg/ml lipopolysaccharide reached the optimal inhibition of 65%. The analysis of mucin secretory responses revealed that the lipopolysaccharide by first 15 min caused a 57% stimulation in sulfomucin secretion followed thereafter by inhibition, which reached maximum of 32% by 45 min. The results suggest that colonization of gastric mucosa by H. pylori may be detrimental to the process of gastric sulfomucin synthesis and secretion.

Original languageEnglish (US)
Pages (from-to)1411-1417
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume184
Issue number3
DOIs
StatePublished - May 15 1992

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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