Background and Aims: Helicobacter pylori alterations in gastric acid output and mucosal proliferation may involve the enterochromaffin-like (ECL) cell. To test whether H. pylori affects ECL cell histamine secretion and proliferation, the effect of lipopolysaccharide (LPS) on ECL cell function in vitro was investigated. Methods: Using a rat ECL cell preparation of high purity (±95%), basal and stimulated histamine secretion and DNA synthesis were measured by enzyme immunoassay and bromodeoxyuridine (BrdU) uptake, respectively. Results: Escherichia coli LPS (10-12 to 10-6 mol/L) had no effect on basal and stimulated histamine secretion at concentrations of >10- 6 mol/L. H. pylori LPS stimulated basal and gastrin-stimulated histamine secretion. These effects were completely inhibited by somatostatin (10-10 mol/L) but not by the gastrin receptor antagonist L365, 260 at 10-6 mol/L. E. coli LPS had at weak stimulatory effect on ECL cell BrdU uptake at 10-6 mol/L but had no effect on gastrin-stimulated BrdU uptake, H. pylori LPS did not stimulate basal synthesis but significantly increased (1.5-fold) gastrin- stimulated BrdU uptake. Conclusions: H. pylori influences both ECL cell proliferation and secretion in vitro. An interaction between H, pylori LPS and ECL cells may contribute to the reported abnormalities in acid secretion and gastric pathobiology noted in H. pylori infections.
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