Hematopoietic growth factor inducible neurokinin-1 type: A transmembrane protein that is similar to neurokinin 1 interacts with substance P

Persis S. Bandari, Jing Qian, Ghassan Yehia, Deval D. Joshi, Paul B. Maloof, Julius Potian, Hyun S. Oh, Pedro Gascon, Jonathan S. Harrison, Pranela Rameshwar

Research output: Contribution to journalArticle

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Abstract

Neurokinin 1 (NK-1) is a member of seven transmembrane G protein-coupled receptors. NK-1 interacts with peptides belonging to the tachykinin family and showed preference for substance P (SP). NK-1 is induced in bone marrow (BM) stroma. NK-1-SP interactions could lead to changes in the functions of lymphohematopoietic stem cell (LHSC). This report describes the cloning and characterization of a cDNA clone isolated after screening of three cDNA libraries with an NK-1-specific probe. Based on its expression, the cDNA clone was designated hematopoietic growth factor inducible neurokinin-1 type (HGFIN). Computational analyses predicted that HGFIN is transmembrane with the carboxyl terminal extracellular. Proteomic studies with purified HGFIN and SP showed noncovalent interactions. HGFIN-SP interactions were supported by transient expression of HGFIN in CHO cells. Transient expression of HGFIN in unstimulated BM fibroblasts led to the induction of endogenous NK-1. Since NK-1 expression in BM fibroblasts requires cell stimulation, these studies suggest that there might be intracellular crosstalk between NK-1 and HGFIN. Northern analyses with total RNA from different BM cell subsets showed that HGFIN was preferentially expressed in differentiated cells. This suggests that HGFIN might be involved in the maturation of LHSC. HGFIN was detected in several other tissues, but not in brain where NK-1 is constitutively expressed.

Original languageEnglish (US)
Pages (from-to)169-178
Number of pages10
JournalRegulatory Peptides
Volume111
Issue number1-3
DOIs
StatePublished - Mar 28 2003

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Substance P
Intercellular Signaling Peptides and Proteins
Proteins
Bone
Bone Marrow
Fibroblasts
Stem cells
Stem Cells
Complementary DNA
Clone Cells
Tachykinins
CHO Cells
Cloning
Crosstalk
Gene Library
Bone Marrow Cells
Proteomics
Organism Cloning
Brain
Screening

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Physiology
  • Endocrinology
  • Clinical Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Bandari, Persis S. ; Qian, Jing ; Yehia, Ghassan ; Joshi, Deval D. ; Maloof, Paul B. ; Potian, Julius ; Oh, Hyun S. ; Gascon, Pedro ; Harrison, Jonathan S. ; Rameshwar, Pranela. / Hematopoietic growth factor inducible neurokinin-1 type : A transmembrane protein that is similar to neurokinin 1 interacts with substance P. In: Regulatory Peptides. 2003 ; Vol. 111, No. 1-3. pp. 169-178.
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abstract = "Neurokinin 1 (NK-1) is a member of seven transmembrane G protein-coupled receptors. NK-1 interacts with peptides belonging to the tachykinin family and showed preference for substance P (SP). NK-1 is induced in bone marrow (BM) stroma. NK-1-SP interactions could lead to changes in the functions of lymphohematopoietic stem cell (LHSC). This report describes the cloning and characterization of a cDNA clone isolated after screening of three cDNA libraries with an NK-1-specific probe. Based on its expression, the cDNA clone was designated hematopoietic growth factor inducible neurokinin-1 type (HGFIN). Computational analyses predicted that HGFIN is transmembrane with the carboxyl terminal extracellular. Proteomic studies with purified HGFIN and SP showed noncovalent interactions. HGFIN-SP interactions were supported by transient expression of HGFIN in CHO cells. Transient expression of HGFIN in unstimulated BM fibroblasts led to the induction of endogenous NK-1. Since NK-1 expression in BM fibroblasts requires cell stimulation, these studies suggest that there might be intracellular crosstalk between NK-1 and HGFIN. Northern analyses with total RNA from different BM cell subsets showed that HGFIN was preferentially expressed in differentiated cells. This suggests that HGFIN might be involved in the maturation of LHSC. HGFIN was detected in several other tissues, but not in brain where NK-1 is constitutively expressed.",
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Hematopoietic growth factor inducible neurokinin-1 type : A transmembrane protein that is similar to neurokinin 1 interacts with substance P. / Bandari, Persis S.; Qian, Jing; Yehia, Ghassan; Joshi, Deval D.; Maloof, Paul B.; Potian, Julius; Oh, Hyun S.; Gascon, Pedro; Harrison, Jonathan S.; Rameshwar, Pranela.

In: Regulatory Peptides, Vol. 111, No. 1-3, 28.03.2003, p. 169-178.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Hematopoietic growth factor inducible neurokinin-1 type

T2 - A transmembrane protein that is similar to neurokinin 1 interacts with substance P

AU - Bandari, Persis S.

AU - Qian, Jing

AU - Yehia, Ghassan

AU - Joshi, Deval D.

AU - Maloof, Paul B.

AU - Potian, Julius

AU - Oh, Hyun S.

AU - Gascon, Pedro

AU - Harrison, Jonathan S.

AU - Rameshwar, Pranela

PY - 2003/3/28

Y1 - 2003/3/28

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