Adult rat hepatocytes cultured in a collagen sandwich system maintained normal morphology and a physiological rtate of albumin secretion for at least 42 days. Hepatocytes cultured on a single layer of collagen gel essentially ceased albumin secretion within 1 wk but could recover function with the overlay of a second layer of collagen gel. This culture configuration more closely mimics the hepatocytes' in vivo environment and provides a simple method for their long-term maintenance.
All Science Journal Classification (ASJC) codes
- Molecular Biology