Hepatocyte function and extracellular matrix geometry: Long-term culture in a sandwich configuration

J. C.Y. Dunn, M. L. Yarmush, H. G. Koebe, R. G. Tompkins

Research output: Contribution to journalArticlepeer-review

633 Scopus citations

Abstract

Adult rat hepatocytes cultured in a collagen sandwich system maintained normal morphology and a physiological rtate of albumin secretion for at least 42 days. Hepatocytes cultured on a single layer of collagen gel essentially ceased albumin secretion within 1 wk but could recover function with the overlay of a second layer of collagen gel. This culture configuration more closely mimics the hepatocytes' in vivo environment and provides a simple method for their long-term maintenance.

Original languageEnglish (US)
Pages (from-to)174-177
Number of pages4
JournalFASEB Journal
Volume3
Issue number2
DOIs
StatePublished - 1989
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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