TY - JOUR
T1 - Heterologous biosynthesis of natural product naringenin by co-culture engineering
AU - Ganesan, Vijaydev
AU - Li, Zhenghong
AU - Wang, Xiaonan
AU - Zhang, Haoran
N1 - Funding Information:
The authors gratefully acknowledge Professor Matthews Koffas (Rensselaer Polytechnic Institute) and Professor Gregory Stephanopoulos (Massachusetts Institute of Technology) for the generous gift of plasmids pOM- Ph CHS- Ms CHI, pCDF-trc- Rg TAL syn - Pc 4CL syn and pCA1 in support of this study. This work is supported by startup research funds from Rutgers, The State University of New Jersey . Zhenghong Li is grateful for the Ph.D. fellowship from the China Scholarship Council .
Funding Information:
The authors gratefully acknowledge Professor Matthews Koffas (Rensselaer Polytechnic Institute) and Professor Gregory Stephanopoulos (Massachusetts Institute of Technology) for the generous gift of plasmids pOM-PhCHS-MsCHI, pCDF-trc-RgTALsyn- Pc4CLsyn and pCA1 in support of this study. This work is supported by startup research funds from Rutgers, The State University of New Jersey. Zhenghong Li is grateful for the Ph.D. fellowship from the China Scholarship Council.
Publisher Copyright:
© 2017 The Authors
PY - 2017/9
Y1 - 2017/9
N2 - Co-culture engineering is an emerging approach for microbial biosynthesis of a variety of biochemicals. In this study, E. coli-E. coli co-cultures were developed for heterologous biosynthesis of the natural product naringenin. The co-cultures were composed of two independent E. coli strains dedicated to functional expression of different portions of the biosynthetic pathway, respectively. The co-culture biosynthesis was optimized by investigating the effect of carbon source, E. coli strain selection, timing of IPTG induction and the inoculation ratio between the co-culture strains. Compared with the mono-culture strategy, the utilization of the designed co-cultures significantly improved the naringenin production, largely due to the reduction of metabolic stress, employment of proper hosts for improving pathway enzyme activities, and flexible adjustment of the relative biosynthetic strength between the co-culture strains. The findings of this study extend the applicability of co-culture engineering in complex natural product biosynthesis.
AB - Co-culture engineering is an emerging approach for microbial biosynthesis of a variety of biochemicals. In this study, E. coli-E. coli co-cultures were developed for heterologous biosynthesis of the natural product naringenin. The co-cultures were composed of two independent E. coli strains dedicated to functional expression of different portions of the biosynthetic pathway, respectively. The co-culture biosynthesis was optimized by investigating the effect of carbon source, E. coli strain selection, timing of IPTG induction and the inoculation ratio between the co-culture strains. Compared with the mono-culture strategy, the utilization of the designed co-cultures significantly improved the naringenin production, largely due to the reduction of metabolic stress, employment of proper hosts for improving pathway enzyme activities, and flexible adjustment of the relative biosynthetic strength between the co-culture strains. The findings of this study extend the applicability of co-culture engineering in complex natural product biosynthesis.
KW - Co-culture engineering
KW - E. coli
KW - Heterologous biosynthesis
KW - Naringenin
UR - http://www.scopus.com/inward/record.url?scp=85035778496&partnerID=8YFLogxK
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U2 - 10.1016/j.synbio.2017.08.003
DO - 10.1016/j.synbio.2017.08.003
M3 - Article
AN - SCOPUS:85035778496
SN - 2405-805X
VL - 2
SP - 236
EP - 242
JO - Synthetic and Systems Biotechnology
JF - Synthetic and Systems Biotechnology
IS - 3
ER -