High mobility group AT-hook 2 regulates osteoblast differentiation and facial bone development

Tsubasa Negishi, Nozomi Mihara, Tadashige Chiba, Jeanine D'Armiento, Kiran Chada, Munehiro Maeda, Masaru Igarashi, Kazushi Imai

Research output: Contribution to journalArticlepeer-review


The mutation and deletion of high mobility group AT-hook 2 (Hmga2) gene exhibit skeletal malformation, but almost nothing is known about the mechanism. This study examined morphological anomaly of facial bone in Hmga2−/− mice and osteoblast differentiation of pre-osteoblast MC3T3-E1 cells with Hmga2 gene knockout (A2KO). Hmga2−/− mice showed the size reduction of anterior frontal part of facial bones. Hmga2 protein and mRNA were expressed in mesenchymal cells at ossification area of nasal bone. A2KO cells differentiation into osteoblasts after reaching the proliferation plateau was strongly suppressed by alizarin red and alkaline phosphatase staining analyses. Expression of osteoblast-related genes, especially Osterix, was down-regulated in A2KO cells. These results demonstrate a close association of Hmga2 with osteoblast differentiation of mesenchymal cells and bone growth. Although future studies are needed, the present study suggests an involvement of Hmga2 in osteoblast-genesis and bone growth.

Original languageEnglish (US)
Pages (from-to)68-74
Number of pages7
JournalBiochemical and Biophysical Research Communications
StatePublished - Jan 29 2022

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


  • Facial bone formation
  • High mobility group AT-hook 2
  • Mouse
  • Osteoblasts


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