High-resolution imaging of bone precursor cells within the intact bone marrow cavity of living mice

Philipp Mayer-Kuckuk; Terence P.F. Gade; Ian M. Buchanan; Mikhail Doubrovin; Ludmilla Ageyeva; Joseph R. Bertino; Adele L. Boskey; Ronald G. Blasberg; Jason A. Koutcher; Debabrata Banerjee

doi:10.1016/j.ymthe.2005.02.018

High-resolution imaging of bone precursor cells within the intact bone marrow cavity of living mice

Philipp Mayer-Kuckuk, Terence P.F. Gade, Ian M. Buchanan, Mikhail Doubrovin, Ludmilla Ageyeva, Joseph R. Bertino, Adele L. Boskey, Ronald G. Blasberg, Jason A. Koutcher, Debabrata Banerjee

Cancer Institute of New Jersey (CINJ), Basic Research

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Bone precursor cells (BPCs) play a critical role in bone maintenance and regeneration. Currently, no tool exists to study BPCs or other bone marrow cell types directly within their complex microenvironment. Here, we describe in vivo magnetic resonance imaging (MRI) of anatomical structures inside the medullary cavity of the mouse femur. We demonstrate that BPCs passively labeled with iron oxide-containing particles can be monitored by MRI within the intact bone marrow at an in-plane resolution of 43 × 25 μm. Anatomical detail provided by MRI is complemented by functional optical imaging of reporter gene expression. Single-cell dual iron oxide-reporter gene labeling has potential for combined cell tracking and cell biology studies. In summary, we describe a versatile platform suitable for studying the biology of many bone marrow cell types in living bone.

Original language	English (US)
Pages (from-to)	33-41
Number of pages	9
Journal	Molecular Therapy
Volume	12
Issue number	1
DOIs	https://doi.org/10.1016/j.ymthe.2005.02.018
State	Published - Jul 2005

All Science Journal Classification (ASJC) codes

Molecular Medicine
Molecular Biology
Genetics
Pharmacology
Drug Discovery

Keywords

Bioluminescence imaging
Bone precursor cell
Cell tracking
Magnetic resonance imaging
Marrow stromal cells
Mesenchymal stem cell
Molecular imaging
Reporter gene

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10.1016/j.ymthe.2005.02.018

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title = "High-resolution imaging of bone precursor cells within the intact bone marrow cavity of living mice",

abstract = "Bone precursor cells (BPCs) play a critical role in bone maintenance and regeneration. Currently, no tool exists to study BPCs or other bone marrow cell types directly within their complex microenvironment. Here, we describe in vivo magnetic resonance imaging (MRI) of anatomical structures inside the medullary cavity of the mouse femur. We demonstrate that BPCs passively labeled with iron oxide-containing particles can be monitored by MRI within the intact bone marrow at an in-plane resolution of 43 × 25 μm. Anatomical detail provided by MRI is complemented by functional optical imaging of reporter gene expression. Single-cell dual iron oxide-reporter gene labeling has potential for combined cell tracking and cell biology studies. In summary, we describe a versatile platform suitable for studying the biology of many bone marrow cell types in living bone.",

keywords = "Bioluminescence imaging, Bone precursor cell, Cell tracking, Magnetic resonance imaging, Marrow stromal cells, Mesenchymal stem cell, Molecular imaging, Reporter gene",

author = "Philipp Mayer-Kuckuk and Gade, {Terence P.F.} and Buchanan, {Ian M.} and Mikhail Doubrovin and Ludmilla Ageyeva and Bertino, {Joseph R.} and Boskey, {Adele L.} and Blasberg, {Ronald G.} and Koutcher, {Jason A.} and Debabrata Banerjee",

note = "Funding Information: This work was funded by NIH Grants P50 CA86438 (D.B., J.A.K., R.G.B.), R24CA83084 (J.A.K.), and CA08748 (J.A.K.). We thank Tatiana Beresten and Roshni Bhakta for help with the animal irradiation and bone formation assay, respectively. Vladimir Ponomarev kindly provided the reporter gene plasmid. Also, we acknowledge the expertise provided by the Flow Cytometry and Molecular Cytology Core Facilities as well as the Research Animal Resource Center at Memorial Sloan–Kettering Cancer Center.",

year = "2005",

month = jul,

doi = "10.1016/j.ymthe.2005.02.018",

language = "English (US)",

volume = "12",

pages = "33--41",

journal = "Molecular Therapy",

issn = "1525-0016",

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number = "1",

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T1 - High-resolution imaging of bone precursor cells within the intact bone marrow cavity of living mice

AU - Mayer-Kuckuk, Philipp

AU - Gade, Terence P.F.

AU - Buchanan, Ian M.

AU - Doubrovin, Mikhail

AU - Ageyeva, Ludmilla

AU - Bertino, Joseph R.

AU - Boskey, Adele L.

AU - Blasberg, Ronald G.

AU - Koutcher, Jason A.

AU - Banerjee, Debabrata

N1 - Funding Information: This work was funded by NIH Grants P50 CA86438 (D.B., J.A.K., R.G.B.), R24CA83084 (J.A.K.), and CA08748 (J.A.K.). We thank Tatiana Beresten and Roshni Bhakta for help with the animal irradiation and bone formation assay, respectively. Vladimir Ponomarev kindly provided the reporter gene plasmid. Also, we acknowledge the expertise provided by the Flow Cytometry and Molecular Cytology Core Facilities as well as the Research Animal Resource Center at Memorial Sloan–Kettering Cancer Center.

PY - 2005/7

Y1 - 2005/7

N2 - Bone precursor cells (BPCs) play a critical role in bone maintenance and regeneration. Currently, no tool exists to study BPCs or other bone marrow cell types directly within their complex microenvironment. Here, we describe in vivo magnetic resonance imaging (MRI) of anatomical structures inside the medullary cavity of the mouse femur. We demonstrate that BPCs passively labeled with iron oxide-containing particles can be monitored by MRI within the intact bone marrow at an in-plane resolution of 43 × 25 μm. Anatomical detail provided by MRI is complemented by functional optical imaging of reporter gene expression. Single-cell dual iron oxide-reporter gene labeling has potential for combined cell tracking and cell biology studies. In summary, we describe a versatile platform suitable for studying the biology of many bone marrow cell types in living bone.

AB - Bone precursor cells (BPCs) play a critical role in bone maintenance and regeneration. Currently, no tool exists to study BPCs or other bone marrow cell types directly within their complex microenvironment. Here, we describe in vivo magnetic resonance imaging (MRI) of anatomical structures inside the medullary cavity of the mouse femur. We demonstrate that BPCs passively labeled with iron oxide-containing particles can be monitored by MRI within the intact bone marrow at an in-plane resolution of 43 × 25 μm. Anatomical detail provided by MRI is complemented by functional optical imaging of reporter gene expression. Single-cell dual iron oxide-reporter gene labeling has potential for combined cell tracking and cell biology studies. In summary, we describe a versatile platform suitable for studying the biology of many bone marrow cell types in living bone.

KW - Bioluminescence imaging

KW - Bone precursor cell

KW - Cell tracking

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KW - Marrow stromal cells

KW - Mesenchymal stem cell

KW - Molecular imaging

KW - Reporter gene

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High-resolution imaging of bone precursor cells within the intact bone marrow cavity of living mice

Abstract

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Keywords

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