Vysokoselektivnoe mechenie kompleksa RNK-polimerazy E. coli s promotorom reaktsionnosposobnymi proizvodnymi oligonukleotidov- zatravok razlichnoǐ spetsifichnosti.

Translated title of the contribution: Highly selective labeling of the E. coli RNA-polymerase promoter complex with reactive derivatives of oligonucleotide primers of various specificity

I. G. Tsarev, A. A. Mustaev, E. F. Zaǐchikov, T. I. Alikina, A. G. Ven'iaminova, M. N. Repkova

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

A technique of highly selective affinity labelling, which includes covalent modification of the enzyme-T7A2 promoter complex with reactive oligonucleotide derivatives and subsequent elongation of the attached oligonucleotide residue with a radioactive substrate was used to study the product-binding site of E. coli RNA polymerase. Different oligonucleotides complementary to the T7A2 promoter (with lengths ranging from 2 to 8 residues) containing 5'-terminal phosphorylating, alkylating or aldehyde groups were used for the labelling. The procedure resulted in labelling DNA and beta-, beta'- or sigma-subunits of the enzyme, which are therefore believed to contact with growing RNA in the course of initiation. Consideration of the labelling patterns as a functions of the oligonucleotide's length as well as of the structure and chemical specificity of the reactive groups led to a tentative topographic scheme of the RNA polymerase product-binding region.

Translated title of the contributionHighly selective labeling of the E. coli RNA-polymerase promoter complex with reactive derivatives of oligonucleotide primers of various specificity
Original languageRussian
Pages (from-to)765-779
Number of pages15
JournalBioorganicheskaia khimiia
Volume16
Issue number6
StatePublished - Jun 1 1990
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Medicine(all)

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