Histone chaperone FACT action during transcription through chromatin by RNA polymerase II

Fu Kai Hsieh, Olga I. Kulaeva, Smita S. Patel, Pamela N. Dyer, Karolin Luger, Danny Reinberg, Vasily M. Studitsky

Research output: Contribution to journalArticlepeer-review

106 Scopus citations


FACT (facilitates chromatin transcription) is a histone chaperone that promotes chromatin recovery during transcription, with additional roles in cell differentiation. Although several models of the action of FACT during transcription have been proposed, they remain to be experimentally evaluated. Here we show that human FACT (hFACT) facilitates transcription through chromatin and promotes nucleosome recovery in vitro. FACT action depends on the presence of histone H2A/H2B dimers in the nucleosome. Kinetic analysis suggests that hFACT decreases the lifetime of nonproductive RNA polymerase II (Pol II)-nucleosome complexes and facilitates the formation of productive complexes containing nucleosomal DNA partially uncoiled from the octamer. Taken together, our data suggest that hFACT interacts with DNA-binding surfaces of H2A/H2B dimers, facilitating uncoiling of DNA from the histone octamer. Thus, hFACT-H2A/ H2B interactions play a key role in overcoming the nucleosomal barrier by Pol II and promoting nucleosome survival during transcription.

Original languageEnglish (US)
Pages (from-to)7654-7659
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number19
StatePublished - May 7 2013

All Science Journal Classification (ASJC) codes

  • General


  • DNA uncoiling
  • Dynamics
  • Elongation
  • Mechanism

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