Human Alveolar T Lymphocyte Responses to Mycobacterium tuberculosis Antigens: Role for CD4+ and CD8+ Cytotoxic T Cells and Relative Resistance of Alveolar Macrophages to Lysis

Jeanette S. Tan, David H. Canaday, W. Henry Boom, Kithiganahalli N. Balaji, Stephan K. Schwander, Elizabeth A. Rich

Research output: Contribution to journalArticlepeer-review

122 Scopus citations

Abstract

T cell-mediated cytotoxicity against Mycobacterium tuberculosis (MTB)-infected macrophages may be a major mechanism of specific host defense, but little is known about such activities in the lung. Thus, the capacity of alveolar lymphocyte MTB-specific cell lines (AL) and alveolar macrophages (AM) from tuberculin skin test-positive healthy subjects to serve as CTL and target cells, respectively, in response to MTB (H37Ra) or purified protein derivative (PPD) was investigated. Mycobacterial Ag-pulsed AM were targets of blood CTL activity at E:T ratios of ≥30:1 (51Cr release assay), but were significantly more resistant to cytotoxicity than autologous blood monocytes. PPD- plus IL-2-expanded AL and blood lymphocytes were cytotoxic for autologous mycobacterium-stimulated monocytes at E:T ratios of ≥10:1. The CTL activity of lymphocytes expanded with PPD was predominantly class II MHC restricted, whereas the CTL activity of lymphocytes expanded with PPD plus IL-2 was both class I and class II MHC restricted. Both CD4+ and CD8+ T cells were enriched in BL and AL expanded with PPD and IL-2, and both subsets had mycobacterium-specific CTL activity. Such novel cytotoxic responses by CD4+ and CD8+ T cells may be a major mechanism of defense against MTB at the site of disease activity.

Original languageEnglish (US)
Pages (from-to)290-297
Number of pages8
JournalJournal of Immunology
Volume159
Issue number1
StatePublished - 1997
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Fingerprint

Dive into the research topics of 'Human Alveolar T Lymphocyte Responses to Mycobacterium tuberculosis Antigens: Role for CD4+ and CD8+ Cytotoxic T Cells and Relative Resistance of Alveolar Macrophages to Lysis'. Together they form a unique fingerprint.

Cite this