Humoral immunity is not critical for protection against experimental infection with Sarcocystis neurona in B-cell-deficient mice

Sharon G. Witonsky, Robert M. Gogal, Robert B. Duncan, Heather Norton, Dan Ward, Jibing Yang, David S. Lindsay

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Immunodeficient B-cell-deficient mice (mμMT) were infected with Sarcocystis neurona merozoites to determine the role of B cells and the humoral immune response in protective immunity. As expected, the mice did not seroconvert based on a direct agglutination test. Infected mice did not have significant changes in gross pathology at the time points examined. Histologic changes included mild perivascular and peribronchial infiltrate in the lungs; perivascular infiltrate and mild inflammatory sinusoidal foci in the liver; prominent high endothelial venules in the lymph nodes; and moderate cellular expansion of the periarteriolar sheaths (PALS) in the spleen. Changes resolved by day 60 postinfection. Mice developed significant CD4 and CD8 responses in lymphoid organs, including significant effector (CD45RBhigh) and memory (CD44high) CD4 and CD8 responses. Flow cytometry confirmed the lack of B cells. Overall, these data suggest that B cells are not critical to the protective immune response to SN infection.

Original languageEnglish (US)
Pages (from-to)830-837
Number of pages8
JournalJournal of Parasitology
Volume91
Issue number4
DOIs
StatePublished - Aug 1 2005

Fingerprint

Sarcocystis
Sarcocystis neurona
Humoral Immunity
immunity
immune response
humoral immunity
B-lymphocytes
B-Lymphocytes
mice
flow cytometry
pathology
Infection
infection
Merozoites
Agglutination Tests
merozoites
Venules
agglutination tests
lymph nodes
Immunity

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Witonsky, Sharon G. ; Gogal, Robert M. ; Duncan, Robert B. ; Norton, Heather ; Ward, Dan ; Yang, Jibing ; Lindsay, David S. / Humoral immunity is not critical for protection against experimental infection with Sarcocystis neurona in B-cell-deficient mice. In: Journal of Parasitology. 2005 ; Vol. 91, No. 4. pp. 830-837.
@article{b278cb5772d14a74ad574a385dcbd293,
title = "Humoral immunity is not critical for protection against experimental infection with Sarcocystis neurona in B-cell-deficient mice",
abstract = "Immunodeficient B-cell-deficient mice (mμMT) were infected with Sarcocystis neurona merozoites to determine the role of B cells and the humoral immune response in protective immunity. As expected, the mice did not seroconvert based on a direct agglutination test. Infected mice did not have significant changes in gross pathology at the time points examined. Histologic changes included mild perivascular and peribronchial infiltrate in the lungs; perivascular infiltrate and mild inflammatory sinusoidal foci in the liver; prominent high endothelial venules in the lymph nodes; and moderate cellular expansion of the periarteriolar sheaths (PALS) in the spleen. Changes resolved by day 60 postinfection. Mice developed significant CD4 and CD8 responses in lymphoid organs, including significant effector (CD45RBhigh) and memory (CD44high) CD4 and CD8 responses. Flow cytometry confirmed the lack of B cells. Overall, these data suggest that B cells are not critical to the protective immune response to SN infection.",
author = "Witonsky, {Sharon G.} and Gogal, {Robert M.} and Duncan, {Robert B.} and Heather Norton and Dan Ward and Jibing Yang and Lindsay, {David S.}",
year = "2005",
month = "8",
day = "1",
doi = "10.1645/GE-488R.1",
language = "English (US)",
volume = "91",
pages = "830--837",
journal = "Journal of Parasitology",
issn = "0022-3395",
publisher = "American Society of Parasitologists",
number = "4",

}

Humoral immunity is not critical for protection against experimental infection with Sarcocystis neurona in B-cell-deficient mice. / Witonsky, Sharon G.; Gogal, Robert M.; Duncan, Robert B.; Norton, Heather; Ward, Dan; Yang, Jibing; Lindsay, David S.

In: Journal of Parasitology, Vol. 91, No. 4, 01.08.2005, p. 830-837.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Humoral immunity is not critical for protection against experimental infection with Sarcocystis neurona in B-cell-deficient mice

AU - Witonsky, Sharon G.

AU - Gogal, Robert M.

AU - Duncan, Robert B.

AU - Norton, Heather

AU - Ward, Dan

AU - Yang, Jibing

AU - Lindsay, David S.

PY - 2005/8/1

Y1 - 2005/8/1

N2 - Immunodeficient B-cell-deficient mice (mμMT) were infected with Sarcocystis neurona merozoites to determine the role of B cells and the humoral immune response in protective immunity. As expected, the mice did not seroconvert based on a direct agglutination test. Infected mice did not have significant changes in gross pathology at the time points examined. Histologic changes included mild perivascular and peribronchial infiltrate in the lungs; perivascular infiltrate and mild inflammatory sinusoidal foci in the liver; prominent high endothelial venules in the lymph nodes; and moderate cellular expansion of the periarteriolar sheaths (PALS) in the spleen. Changes resolved by day 60 postinfection. Mice developed significant CD4 and CD8 responses in lymphoid organs, including significant effector (CD45RBhigh) and memory (CD44high) CD4 and CD8 responses. Flow cytometry confirmed the lack of B cells. Overall, these data suggest that B cells are not critical to the protective immune response to SN infection.

AB - Immunodeficient B-cell-deficient mice (mμMT) were infected with Sarcocystis neurona merozoites to determine the role of B cells and the humoral immune response in protective immunity. As expected, the mice did not seroconvert based on a direct agglutination test. Infected mice did not have significant changes in gross pathology at the time points examined. Histologic changes included mild perivascular and peribronchial infiltrate in the lungs; perivascular infiltrate and mild inflammatory sinusoidal foci in the liver; prominent high endothelial venules in the lymph nodes; and moderate cellular expansion of the periarteriolar sheaths (PALS) in the spleen. Changes resolved by day 60 postinfection. Mice developed significant CD4 and CD8 responses in lymphoid organs, including significant effector (CD45RBhigh) and memory (CD44high) CD4 and CD8 responses. Flow cytometry confirmed the lack of B cells. Overall, these data suggest that B cells are not critical to the protective immune response to SN infection.

UR - http://www.scopus.com/inward/record.url?scp=24944501203&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=24944501203&partnerID=8YFLogxK

U2 - 10.1645/GE-488R.1

DO - 10.1645/GE-488R.1

M3 - Article

C2 - 17089750

AN - SCOPUS:24944501203

VL - 91

SP - 830

EP - 837

JO - Journal of Parasitology

JF - Journal of Parasitology

SN - 0022-3395

IS - 4

ER -