Abstract
Immunodeficient B-cell-deficient mice (mμMT) were infected with Sarcocystis neurona merozoites to determine the role of B cells and the humoral immune response in protective immunity. As expected, the mice did not seroconvert based on a direct agglutination test. Infected mice did not have significant changes in gross pathology at the time points examined. Histologic changes included mild perivascular and peribronchial infiltrate in the lungs; perivascular infiltrate and mild inflammatory sinusoidal foci in the liver; prominent high endothelial venules in the lymph nodes; and moderate cellular expansion of the periarteriolar sheaths (PALS) in the spleen. Changes resolved by day 60 postinfection. Mice developed significant CD4 and CD8 responses in lymphoid organs, including significant effector (CD45RBhigh) and memory (CD44high) CD4 and CD8 responses. Flow cytometry confirmed the lack of B cells. Overall, these data suggest that B cells are not critical to the protective immune response to SN infection.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 830-837 |
| Number of pages | 8 |
| Journal | Journal of Parasitology |
| Volume | 91 |
| Issue number | 4 |
| DOIs | |
| State | Published - Aug 1 2005 |
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All Science Journal Classification (ASJC) codes
- Parasitology
- Ecology, Evolution, Behavior and Systematics
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Humoral immunity is not critical for protection against experimental infection with Sarcocystis neurona in B-cell-deficient mice. / Witonsky, Sharon G.; Gogal, Robert M.; Duncan, Robert B.; Norton, Heather; Ward, Dan; Yang, Jibing; Lindsay, David S.
In: Journal of Parasitology, Vol. 91, No. 4, 01.08.2005, p. 830-837.Research output: Contribution to journal › Article
TY - JOUR
T1 - Humoral immunity is not critical for protection against experimental infection with Sarcocystis neurona in B-cell-deficient mice
AU - Witonsky, Sharon G.
AU - Gogal, Robert M.
AU - Duncan, Robert B.
AU - Norton, Heather
AU - Ward, Dan
AU - Yang, Jibing
AU - Lindsay, David S.
PY - 2005/8/1
Y1 - 2005/8/1
N2 - Immunodeficient B-cell-deficient mice (mμMT) were infected with Sarcocystis neurona merozoites to determine the role of B cells and the humoral immune response in protective immunity. As expected, the mice did not seroconvert based on a direct agglutination test. Infected mice did not have significant changes in gross pathology at the time points examined. Histologic changes included mild perivascular and peribronchial infiltrate in the lungs; perivascular infiltrate and mild inflammatory sinusoidal foci in the liver; prominent high endothelial venules in the lymph nodes; and moderate cellular expansion of the periarteriolar sheaths (PALS) in the spleen. Changes resolved by day 60 postinfection. Mice developed significant CD4 and CD8 responses in lymphoid organs, including significant effector (CD45RBhigh) and memory (CD44high) CD4 and CD8 responses. Flow cytometry confirmed the lack of B cells. Overall, these data suggest that B cells are not critical to the protective immune response to SN infection.
AB - Immunodeficient B-cell-deficient mice (mμMT) were infected with Sarcocystis neurona merozoites to determine the role of B cells and the humoral immune response in protective immunity. As expected, the mice did not seroconvert based on a direct agglutination test. Infected mice did not have significant changes in gross pathology at the time points examined. Histologic changes included mild perivascular and peribronchial infiltrate in the lungs; perivascular infiltrate and mild inflammatory sinusoidal foci in the liver; prominent high endothelial venules in the lymph nodes; and moderate cellular expansion of the periarteriolar sheaths (PALS) in the spleen. Changes resolved by day 60 postinfection. Mice developed significant CD4 and CD8 responses in lymphoid organs, including significant effector (CD45RBhigh) and memory (CD44high) CD4 and CD8 responses. Flow cytometry confirmed the lack of B cells. Overall, these data suggest that B cells are not critical to the protective immune response to SN infection.
UR - http://www.scopus.com/inward/record.url?scp=24944501203&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=24944501203&partnerID=8YFLogxK
U2 - 10.1645/GE-488R.1
DO - 10.1645/GE-488R.1
M3 - Article
C2 - 17089750
AN - SCOPUS:24944501203
VL - 91
SP - 830
EP - 837
JO - Journal of Parasitology
JF - Journal of Parasitology
SN - 0022-3395
IS - 4
ER -