HuR posttranscriptionally regulates early growth response-1 (Egr-1) expression at the early stage of T cell activation

Zongchun Mou, Jia You, Qianghai Xiao, Youheng Wei, Juan Yuan, Yong Liu, Gary Brewer, Wei Jun Ma

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

T cell activation depends on appropriate and precise regulation of gene expression. Here we find that rapidly translocated RNA-binding protein HuR, forms messenger ribonucleoprotein (mRNP) complexes with transiently expressed mRNAs encoding early-response transcription factors, including c-Fos, c-Jun, and Egr-1. Knockdown and overexpression assays demonstrated that proper posttranscriptional control of Egr-1 expression requires HuR-mediated translation control. Further analysis showed that the Egr-1 3′UTR, which contains AU-rich elements (AREs) and interacts directly with HuR, suppresses reporter gene expression and mediates posttranscriptional regulation of Egr-1 by HuR. These findings underscore an essential role for HuR in regulating early events during T cell activation.

Original languageEnglish (US)
Pages (from-to)4319-4325
Number of pages7
JournalFEBS Letters
Volume586
Issue number24
DOIs
StatePublished - Dec 14 2012
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Keywords

  • 3′UTR
  • Egr-1
  • HuR
  • Posttranscriptional regulation
  • T cell activation
  • Translation

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