Hydrophobic clustering in acid-denatured IL-2 and fluorescence of a Trp NH···π H-bond

Vikas Nanda, Shu Mei Liang, Ludwig Brand

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The single tryptophan at position 121 of human interleukin-2 (IL-2) can form an NH···π hydrogen bond with Phe 117 involving the indole nitrogen and the benzene aromatic ring. At pH 5.5, this type of aromatic interaction results in a fluorescence quantum yield three-fold lower than that of a fully solvent exposed tryptophan. At pH 2.1, IL-2 forms a compact denatured state with twice the emission intensity of the native protein. Global analysis of time-resolved fluorescence emission at multiple emission wavelengths shows that native and acid-denatured IL-2 can be described by four decay components. The fractional amplitudes of the shortest sub-nanosecond lifetimes are higher in the native state, suggesting rapid quenching due to the NH···π hydrogen bond. In the denatured state, longer lifetimes have greater fractional amplitudes, indicating a smaller population of hydrogen-bonded species. Electrostaticdipolar relaxation of the tryptophan microenvironment upon excitation is greater in the native-state of IL-2 than the acid-denatured state. This suggests that aciddenaturation sequesters Trp 121 from polar residues, while maintaining an interaction with Phe 117. This is consistent with the model of secondary structure preservation and hydrophobic clustering in molten-globule intermediates.

Original languageEnglish (US)
Pages (from-to)770-778
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume279
Issue number3
DOIs
StatePublished - Dec 29 2000

Fingerprint

Interleukin-2
Cluster Analysis
Fluorescence
Tryptophan
Hydrogen
Acids
Hydrogen bonds
Rapid quenching
Quantum yield
Benzene
Molten materials
Nitrogen
Wavelength
Population
Proteins

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Keywords

  • Dielectric relaxation
  • Heterogeneity
  • Molten globule
  • Time-resolved fluorescence
  • Tryptophan

Cite this

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title = "Hydrophobic clustering in acid-denatured IL-2 and fluorescence of a Trp NH···π H-bond",
abstract = "The single tryptophan at position 121 of human interleukin-2 (IL-2) can form an NH···π hydrogen bond with Phe 117 involving the indole nitrogen and the benzene aromatic ring. At pH 5.5, this type of aromatic interaction results in a fluorescence quantum yield three-fold lower than that of a fully solvent exposed tryptophan. At pH 2.1, IL-2 forms a compact denatured state with twice the emission intensity of the native protein. Global analysis of time-resolved fluorescence emission at multiple emission wavelengths shows that native and acid-denatured IL-2 can be described by four decay components. The fractional amplitudes of the shortest sub-nanosecond lifetimes are higher in the native state, suggesting rapid quenching due to the NH···π hydrogen bond. In the denatured state, longer lifetimes have greater fractional amplitudes, indicating a smaller population of hydrogen-bonded species. Electrostaticdipolar relaxation of the tryptophan microenvironment upon excitation is greater in the native-state of IL-2 than the acid-denatured state. This suggests that aciddenaturation sequesters Trp 121 from polar residues, while maintaining an interaction with Phe 117. This is consistent with the model of secondary structure preservation and hydrophobic clustering in molten-globule intermediates.",
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Hydrophobic clustering in acid-denatured IL-2 and fluorescence of a Trp NH···π H-bond. / Nanda, Vikas; Liang, Shu Mei; Brand, Ludwig.

In: Biochemical and Biophysical Research Communications, Vol. 279, No. 3, 29.12.2000, p. 770-778.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Hydrophobic clustering in acid-denatured IL-2 and fluorescence of a Trp NH···π H-bond

AU - Nanda, Vikas

AU - Liang, Shu Mei

AU - Brand, Ludwig

PY - 2000/12/29

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N2 - The single tryptophan at position 121 of human interleukin-2 (IL-2) can form an NH···π hydrogen bond with Phe 117 involving the indole nitrogen and the benzene aromatic ring. At pH 5.5, this type of aromatic interaction results in a fluorescence quantum yield three-fold lower than that of a fully solvent exposed tryptophan. At pH 2.1, IL-2 forms a compact denatured state with twice the emission intensity of the native protein. Global analysis of time-resolved fluorescence emission at multiple emission wavelengths shows that native and acid-denatured IL-2 can be described by four decay components. The fractional amplitudes of the shortest sub-nanosecond lifetimes are higher in the native state, suggesting rapid quenching due to the NH···π hydrogen bond. In the denatured state, longer lifetimes have greater fractional amplitudes, indicating a smaller population of hydrogen-bonded species. Electrostaticdipolar relaxation of the tryptophan microenvironment upon excitation is greater in the native-state of IL-2 than the acid-denatured state. This suggests that aciddenaturation sequesters Trp 121 from polar residues, while maintaining an interaction with Phe 117. This is consistent with the model of secondary structure preservation and hydrophobic clustering in molten-globule intermediates.

AB - The single tryptophan at position 121 of human interleukin-2 (IL-2) can form an NH···π hydrogen bond with Phe 117 involving the indole nitrogen and the benzene aromatic ring. At pH 5.5, this type of aromatic interaction results in a fluorescence quantum yield three-fold lower than that of a fully solvent exposed tryptophan. At pH 2.1, IL-2 forms a compact denatured state with twice the emission intensity of the native protein. Global analysis of time-resolved fluorescence emission at multiple emission wavelengths shows that native and acid-denatured IL-2 can be described by four decay components. The fractional amplitudes of the shortest sub-nanosecond lifetimes are higher in the native state, suggesting rapid quenching due to the NH···π hydrogen bond. In the denatured state, longer lifetimes have greater fractional amplitudes, indicating a smaller population of hydrogen-bonded species. Electrostaticdipolar relaxation of the tryptophan microenvironment upon excitation is greater in the native-state of IL-2 than the acid-denatured state. This suggests that aciddenaturation sequesters Trp 121 from polar residues, while maintaining an interaction with Phe 117. This is consistent with the model of secondary structure preservation and hydrophobic clustering in molten-globule intermediates.

KW - Dielectric relaxation

KW - Heterogeneity

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KW - Time-resolved fluorescence

KW - Tryptophan

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