Hypoglycemic activity of Chenopodium formosanum Koidz. components using a glucose uptake assay with 3T3-L1 adipocytes

Bo Yang Hsu, Shih Ying Pan, Liang Yi Wu, Chi Tang Ho, Lucy Sun Hwang

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Djulis (Chenopodium formosanum Koidz.) is a cereal plant native to Taiwan. In this study, the hypoglycemic activity of djulis was investigated using a glucose uptake assay with 3T3-L1 adipocytes. Dehusk seeds (SD) or husks (HD) of djulis were extracted using 6 solvents with different polarities (i.e., water, hexane, acetone, ethyl acetate, methanol and ethanol). The crude water extract of husk of djulis (WHD) showed the highest increase in glucose uptake with 3T3-L1 adipocytes. Thus, it was further separated by solvent partition, XAD-7 gel column chromatography, and HPLC. Two hypoglycemic components (P1 and P2) were isolated from WHD. They showed hypoglycemic activity at 5–10 ppm. P1 might have a synergistic effect with insulin. P2, however, did not enhance insulin-stimulated glucose uptake, which might mean it is an insulin mimic. The results suggested that djulis is a potential anti-diabetes functional food ingredient.

Original languageEnglish (US)
Pages (from-to)9-16
Number of pages8
JournalFood Bioscience
Volume24
DOIs
StatePublished - Aug 2018

All Science Journal Classification (ASJC) codes

  • Food Science
  • Biochemistry

Keywords

  • 3T3-L1 adipocytes
  • Chenopodium formosanum
  • Diabetes
  • Djulis
  • Glucose uptake
  • Hypoglycemic

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