Hypolipidemic activity of Taraxacum mongolicum associated with the activation of AMP-activated protein kinase in human HepG2 cells

Yan Jin Liu, Po Chuen Shieh, Jang Chang Lee, Fu An Chen, Chih Hung Lee, Sheng Chu Kuo, Chi Tang Ho, Daih Huang Kuo, Li Jiau Huang, Tzong Der Way

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Abstract

This study investigated the hypolipidemic effect and potential mechanisms of T. mongolicum extracts. T. mongolicum was extracted by refluxing three times with water (TM-1), 50% ethanol (TM-2) and 95% ethanol (TM-3). TM-2 contained components with the most effective hypolipidemic potentials in HepG2 cells. Extended administration of TM-2 stimulated a significant reduction in body weight and levels of serum triglyceride LDL-C and total cholesterol in rats. To evaluate the bioactive compounds, we successively fractionated TM-2 with n-hexane (TM-4), dichloromethane (TM-5), ethyl acetate (TM-6), and water (TM-7). TM-4 fraction had the most effective hypolipidemic potential in HepG2 cells, and it decreased the expression of fatty acid synthase (FASN) and inhibited the activity of acetyl-coenzyme A carboxylase (ACC) through the phosphorylation of AMP-activated protein kinase (AMPK). Linoleic acid, phytol and tetracosanol are bioactive compounds identified from TM-4. These results suggest that T. mongolicum is expected to be useful for hypolipidemic effects.

Original languageEnglish (US)
Pages (from-to)1755-1762
Number of pages8
JournalFood and Function
Volume5
Issue number8
DOIs
StatePublished - Aug 2014

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All Science Journal Classification (ASJC) codes

  • Food Science

Cite this

Liu, Y. J., Shieh, P. C., Lee, J. C., Chen, F. A., Lee, C. H., Kuo, S. C., Ho, C. T., Kuo, D. H., Huang, L. J., & Way, T. D. (2014). Hypolipidemic activity of Taraxacum mongolicum associated with the activation of AMP-activated protein kinase in human HepG2 cells. Food and Function, 5(8), 1755-1762. https://doi.org/10.1039/c4fo00183d