Hypoxia inducible factor-alpha activation in lymphoma and relationship to the thioredoxin family

Andrew Evens, Paul T. Schumacker, Irene B. Helenowski, Amareshwar T K Singh, Danijela Dokic, Anjeni Keswani, Elizabeth Kordeluk, Adekunle Raji, Jane N. Winter, Borko D. Jovanovic, Arne Holmgren, Beverly P. Nelson, Leo I. Gordon

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Hypoxia inducible factors (HIFs) activate oncogenic pathways, while thioredoxins (Trx), including Trx1 and Trx reductases-1 and -2 (TrxR1 and TrxR2), promote HIF-α stabilization. In immunoblotting studies in lymphoma cell lines we found that Raji and SUDHL4 cells exhibited normoxic HIF-2α protein stabilization. Five cell lines showed increased TrxR1 expression, while only Namalwa, HF1 and SUDHL4 had Trx1 and TrxR2 activation. Tissue microarrays in diffuse large B-cell lymphoma (DLBCL) and follicular lymphoma (FL) identified different HIF expression among histological subgroups (e.g. 44% DLBCL vs. 11% of FL cases with moderate-to-high expression of HIF-1α and HIF-2α, P = 0.0017). These data demonstrate that HIF and the thioredoxin family are abnormally activated in lymphoma.

Original languageEnglish (US)
Pages (from-to)676-680
Number of pages5
JournalBritish Journal of Haematology
Volume141
Issue number5
DOIs
StatePublished - Jun 1 2008

Fingerprint

Thioredoxins
Lymphoma
Follicular Lymphoma
Lymphoma, Large B-Cell, Diffuse
Thioredoxin Reductase 2
Thioredoxin Reductase 1
Hypoxia-Inducible Factor 1
Cell Line
Immunoblotting
Hypoxia
Proteins
endothelial PAS domain-containing protein 1

All Science Journal Classification (ASJC) codes

  • Hematology

Keywords

  • Hypoxia inducible factor
  • Lymphoma
  • Prognosis
  • Thioredoxin
  • Tissue microarray

Cite this

Evens, A., Schumacker, P. T., Helenowski, I. B., Singh, A. T. K., Dokic, D., Keswani, A., ... Gordon, L. I. (2008). Hypoxia inducible factor-alpha activation in lymphoma and relationship to the thioredoxin family. British Journal of Haematology, 141(5), 676-680. https://doi.org/10.1111/j.1365-2141.2008.07093.x
Evens, Andrew ; Schumacker, Paul T. ; Helenowski, Irene B. ; Singh, Amareshwar T K ; Dokic, Danijela ; Keswani, Anjeni ; Kordeluk, Elizabeth ; Raji, Adekunle ; Winter, Jane N. ; Jovanovic, Borko D. ; Holmgren, Arne ; Nelson, Beverly P. ; Gordon, Leo I. / Hypoxia inducible factor-alpha activation in lymphoma and relationship to the thioredoxin family. In: British Journal of Haematology. 2008 ; Vol. 141, No. 5. pp. 676-680.
@article{7ab896519416487e8d3ef1719c34795d,
title = "Hypoxia inducible factor-alpha activation in lymphoma and relationship to the thioredoxin family",
abstract = "Hypoxia inducible factors (HIFs) activate oncogenic pathways, while thioredoxins (Trx), including Trx1 and Trx reductases-1 and -2 (TrxR1 and TrxR2), promote HIF-α stabilization. In immunoblotting studies in lymphoma cell lines we found that Raji and SUDHL4 cells exhibited normoxic HIF-2α protein stabilization. Five cell lines showed increased TrxR1 expression, while only Namalwa, HF1 and SUDHL4 had Trx1 and TrxR2 activation. Tissue microarrays in diffuse large B-cell lymphoma (DLBCL) and follicular lymphoma (FL) identified different HIF expression among histological subgroups (e.g. 44{\%} DLBCL vs. 11{\%} of FL cases with moderate-to-high expression of HIF-1α and HIF-2α, P = 0.0017). These data demonstrate that HIF and the thioredoxin family are abnormally activated in lymphoma.",
keywords = "Hypoxia inducible factor, Lymphoma, Prognosis, Thioredoxin, Tissue microarray",
author = "Andrew Evens and Schumacker, {Paul T.} and Helenowski, {Irene B.} and Singh, {Amareshwar T K} and Danijela Dokic and Anjeni Keswani and Elizabeth Kordeluk and Adekunle Raji and Winter, {Jane N.} and Jovanovic, {Borko D.} and Arne Holmgren and Nelson, {Beverly P.} and Gordon, {Leo I.}",
year = "2008",
month = "6",
day = "1",
doi = "10.1111/j.1365-2141.2008.07093.x",
language = "English (US)",
volume = "141",
pages = "676--680",
journal = "British Journal of Haematology",
issn = "0007-1048",
publisher = "Wiley-Blackwell",
number = "5",

}

Evens, A, Schumacker, PT, Helenowski, IB, Singh, ATK, Dokic, D, Keswani, A, Kordeluk, E, Raji, A, Winter, JN, Jovanovic, BD, Holmgren, A, Nelson, BP & Gordon, LI 2008, 'Hypoxia inducible factor-alpha activation in lymphoma and relationship to the thioredoxin family', British Journal of Haematology, vol. 141, no. 5, pp. 676-680. https://doi.org/10.1111/j.1365-2141.2008.07093.x

Hypoxia inducible factor-alpha activation in lymphoma and relationship to the thioredoxin family. / Evens, Andrew; Schumacker, Paul T.; Helenowski, Irene B.; Singh, Amareshwar T K; Dokic, Danijela; Keswani, Anjeni; Kordeluk, Elizabeth; Raji, Adekunle; Winter, Jane N.; Jovanovic, Borko D.; Holmgren, Arne; Nelson, Beverly P.; Gordon, Leo I.

In: British Journal of Haematology, Vol. 141, No. 5, 01.06.2008, p. 676-680.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Hypoxia inducible factor-alpha activation in lymphoma and relationship to the thioredoxin family

AU - Evens, Andrew

AU - Schumacker, Paul T.

AU - Helenowski, Irene B.

AU - Singh, Amareshwar T K

AU - Dokic, Danijela

AU - Keswani, Anjeni

AU - Kordeluk, Elizabeth

AU - Raji, Adekunle

AU - Winter, Jane N.

AU - Jovanovic, Borko D.

AU - Holmgren, Arne

AU - Nelson, Beverly P.

AU - Gordon, Leo I.

PY - 2008/6/1

Y1 - 2008/6/1

N2 - Hypoxia inducible factors (HIFs) activate oncogenic pathways, while thioredoxins (Trx), including Trx1 and Trx reductases-1 and -2 (TrxR1 and TrxR2), promote HIF-α stabilization. In immunoblotting studies in lymphoma cell lines we found that Raji and SUDHL4 cells exhibited normoxic HIF-2α protein stabilization. Five cell lines showed increased TrxR1 expression, while only Namalwa, HF1 and SUDHL4 had Trx1 and TrxR2 activation. Tissue microarrays in diffuse large B-cell lymphoma (DLBCL) and follicular lymphoma (FL) identified different HIF expression among histological subgroups (e.g. 44% DLBCL vs. 11% of FL cases with moderate-to-high expression of HIF-1α and HIF-2α, P = 0.0017). These data demonstrate that HIF and the thioredoxin family are abnormally activated in lymphoma.

AB - Hypoxia inducible factors (HIFs) activate oncogenic pathways, while thioredoxins (Trx), including Trx1 and Trx reductases-1 and -2 (TrxR1 and TrxR2), promote HIF-α stabilization. In immunoblotting studies in lymphoma cell lines we found that Raji and SUDHL4 cells exhibited normoxic HIF-2α protein stabilization. Five cell lines showed increased TrxR1 expression, while only Namalwa, HF1 and SUDHL4 had Trx1 and TrxR2 activation. Tissue microarrays in diffuse large B-cell lymphoma (DLBCL) and follicular lymphoma (FL) identified different HIF expression among histological subgroups (e.g. 44% DLBCL vs. 11% of FL cases with moderate-to-high expression of HIF-1α and HIF-2α, P = 0.0017). These data demonstrate that HIF and the thioredoxin family are abnormally activated in lymphoma.

KW - Hypoxia inducible factor

KW - Lymphoma

KW - Prognosis

KW - Thioredoxin

KW - Tissue microarray

UR - http://www.scopus.com/inward/record.url?scp=43449116075&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=43449116075&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2141.2008.07093.x

DO - 10.1111/j.1365-2141.2008.07093.x

M3 - Article

C2 - 18422776

AN - SCOPUS:43449116075

VL - 141

SP - 676

EP - 680

JO - British Journal of Haematology

JF - British Journal of Haematology

SN - 0007-1048

IS - 5

ER -