Identification and characterization of a rat novel gene RSEP4 expressed specifically in central nervous system

Xi Dao Wang, Ling Wei Kong, Zhi Qin Xie, Yu Qiu Zhang, Zhi Xin Lin, Zhi Qi Zhao, Lei Yu, Nai He Jing

Research output: Contribution to journalArticlepeer-review

Abstract

The low-abundantly expressed genes composed the majorities of the mRNAs expressed in the central nervous system (CNS), and were thought to be important for the normal brain functions. Through differential screening a low-abundance cDNA sublibrary with mRNA from neuropathic pain of chronic constriction injury (CCI) model, we have identified a novel rat gene, rat spinal-cord expression protein 4 gene (RSEP4). The total length of RSEP4 cDNA is 2006 bp, with a 501 nucleotide open reading frame (ORF) that encodes a 167 amino acid polypeptide. Northern blot revealed that RSEP4 was expressed specifically in the CNS. In situ hybridization showed that the mRNA of RSEP4 was strongly expressed in the CA1, CA2, CA3 and DG regions of hippocampus, the Purkinje cells of cerebellum, and the small sensory neurons of dorsal horn and large motor neurons of ventral horn of spinal cord. Over-expression of RSEP4-EGFP fusion protein in the human embryonic kidney 293T cells showed that RSEP4 protein was mainly localized in the cell cytoplasm. These results suggest that RSEP4 may play some roles in the CNS.

Original languageEnglish (US)
Pages (from-to)501-507
Number of pages7
JournalActa Biochimica et Biophysica Sinica
Volume36
Issue number7
DOIs
StatePublished - Jul 2004
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry

Keywords

  • Central nervous system (CNS)
  • In situ hybridization
  • Northern blot
  • Rat

Fingerprint

Dive into the research topics of 'Identification and characterization of a rat novel gene RSEP4 expressed specifically in central nervous system'. Together they form a unique fingerprint.

Cite this