Identification and characterization of five cspA homologous genes from Myxococcus xanthus

Kunitoshi Yamanaka, Masayori Inouye, Sumiko Inouye

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Escherichia coli contains a large CspA family consisting of nine homologues, in which four are cold-shock inducible and one is stationary-phase inducible. Here, we demonstrate that Myxococcus xanthus possesses at least five CspA homologues, CspA to CspE. Hydrophobic residues forming a hydrophobic core, and aromatic residues, which are included in functional motifs RNP-1 and RNP-2 involved in binding to RNA and ssDNA, are well conserved. These facts suggest that M. xanthus CspA homologues have a similar structure and function as E. coli CspA. However, in contrast to the E. coli CspA family, the expression of M. xanthus csp genes as judged by primer extension analysis is not significantly regulated by temperature changes, except for cspB of which expression was reduced to less than 10% upon heat shock at 42°C. Such constitutive expression of the csp genes may be important for M. xanthus, a soil-dwelling bacterium, to survive under conditions of exposure to various environmental changes in nature. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)357-365
Number of pages9
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1447
Issue number2-3
DOIs
StatePublished - Oct 28 1999

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

Keywords

  • Cold shock
  • CspA family
  • Gene expression
  • Myxococcus xanthus
  • RNA binding motif

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