TY - JOUR
T1 - Identification and evolution of an IS6110 low-copy-number Mycobacterium tuberculosis cluster
AU - Mathema, Barun
AU - Bifani, Pablo J.
AU - Driscoll, Jeffrey
AU - Steinlein, Lauren
AU - Kurepina, Natalia
AU - Moghazeh, Soraya L.
AU - Shashkina, Elena
AU - Marras, Salvatore A.
AU - Campbell, Shannon
AU - Mangura, Bonita
AU - Shilkret, Kenneth
AU - Crawford, Jack T.
AU - Frothingham, Richard
AU - Kreiswirth, Barry N.
PY - 2002/3/1
Y1 - 2002/3/1
N2 - A cohort of 56 patients infected with related strains of Mycobacterium tuberculosis, the S75 group, was identified in a New Jersey population - based study of all isolates with a low number of copies of the insertion element IS6110. Genotyping was combined with surveillance data to identify the S75 group and to elucidate its recent evolution. The S75 group had similar demographic and geographic characteristics. Seventeen persons (30%) were linked epidemiologically. The S75 group was segregated from other low-copy-number isolates on the basis of several independent molecular methods. This group included 3 IS6110 genotype variants: BE, H6, and C28, containing 1, 2, and 3 IS6110 insertions, respectively. IS6110 insertion site mapping and comparative sequence analysis strongly suggest a stepwise acquisition of IS6110 elements from BE to H6 to C28. S75 represents a locally produced strain cluster that has recently evolved. The combination of multiple molecular tools with traditional epidemiology provides novel insights into dissemination, local transmission, and evolution of M. tuberculosis.
AB - A cohort of 56 patients infected with related strains of Mycobacterium tuberculosis, the S75 group, was identified in a New Jersey population - based study of all isolates with a low number of copies of the insertion element IS6110. Genotyping was combined with surveillance data to identify the S75 group and to elucidate its recent evolution. The S75 group had similar demographic and geographic characteristics. Seventeen persons (30%) were linked epidemiologically. The S75 group was segregated from other low-copy-number isolates on the basis of several independent molecular methods. This group included 3 IS6110 genotype variants: BE, H6, and C28, containing 1, 2, and 3 IS6110 insertions, respectively. IS6110 insertion site mapping and comparative sequence analysis strongly suggest a stepwise acquisition of IS6110 elements from BE to H6 to C28. S75 represents a locally produced strain cluster that has recently evolved. The combination of multiple molecular tools with traditional epidemiology provides novel insights into dissemination, local transmission, and evolution of M. tuberculosis.
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U2 - 10.1086/339345
DO - 10.1086/339345
M3 - Article
C2 - 11865421
AN - SCOPUS:0036498747
SN - 0022-1899
VL - 185
SP - 641
EP - 649
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 5
ER -