Identification and Massively Parallel Characterization of Regulatory Elements Driving Neural Induction

Fumitaka Inoue, Anat Kreimer, Tal Ashuach, Nadav Ahituv, Nir Yosef

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Epigenomic regulation and lineage-specific gene expression act in concert to drive cellular differentiation, but the temporal interplay between these processes is largely unknown. Using neural induction from human pluripotent stem cells (hPSCs) as a paradigm, we interrogated these dynamics by performing RNA sequencing (RNA-seq), chromatin immunoprecipitation sequencing (ChIP-seq), and assay for transposase accessible chromatin using sequencing (ATAC-seq) at seven time points during early neural differentiation. We found that changes in DNA accessibility precede H3K27ac, which is followed by gene expression changes. Using massively parallel reporter assays (MPRAs) to test the activity of 2,464 candidate regulatory sequences at all seven time points, we show that many of these sequences have temporal activity patterns that correlate with their respective cell-endogenous gene expression and chromatin changes. A prioritization method incorporating all genomic and MPRA data further identified key transcription factors involved in driving neural fate. These results provide a comprehensive resource of genes and regulatory elements that orchestrate neural induction and illuminate temporal frameworks during differentiation.

Original languageEnglish (US)
Pages (from-to)713-727.e10
JournalCell Stem Cell
Volume25
Issue number5
DOIs
StatePublished - Nov 7 2019
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Genetics
  • Cell Biology

Keywords

  • ATAC-seq
  • ChIP-seq
  • RNA-seq
  • enhancer
  • functional genomics
  • genetic variation
  • massively parallel reporter assay
  • neural induction
  • stem cell differentiation
  • transcriptional regulation

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