Identification of a Mg2+-sensitive ORF in the 5-leader of TRPM7 magnesium channel mRNA

Inna A. Nikonorova, Nikolay V. Kornakov, Sergey E. Dmitriev, Konstantin S. Vassilenko, Alexey G. Ryazanov

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

TRPM7 is an essential and ubiquitous channel-kinase regulating cellular influx of Mg2+. Although TRPM7 mRNA is highly abundant, very small amount of the protein is detected in cells, suggesting post-transcriptional regulation of trpm7 gene expression. We found that TRPM7 mRNA 5′-leader contains two evolutionarily conserved upstream open reading frames that act together to drastically inhibit translation of the TRPM7 reading frame at high magnesium levels and ensure its optimal translation at low magnesium levels, when the activity of the channel-kinase is most required. The study provides the first example of magnesium channel synthesis being controlled by Mg2+ in higher eukaryotes.

Original languageEnglish (US)
Pages (from-to)12779-12788
Number of pages10
JournalNucleic acids research
Volume42
Issue number20
DOIs
StatePublished - Oct 17 2014

All Science Journal Classification (ASJC) codes

  • Genetics

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