TY - JOUR
T1 - Identification of a novel alternative splicing form of human netrin-4 and analyzing the expression patterns in adult rat brain
AU - Zhang, Chenggang
AU - Meng, Fanwei
AU - Wang, Chunli
AU - Guo, Huiyun
AU - Fan, Ming
AU - Liu, Shaojun
AU - Zhou, Renping
AU - He, Fuchu
N1 - Funding Information:
This work was supported by the National Basic Research Project (973 program) (2003CB715900); National High Technology Research and Development Program of China (863 Program) (2002AA234021, 2002AA832031); Joint Research Fund for Overseas Chinese Young Scholars (30128010), Major Program (30393130), Major Research Plan (90208017) and General Program (30300358, 30200154, 30100049) of National Natural Science Foundation of China; Major Project Plan of Science and Technique Plan of Beijing, China (H020220020510) and General Program of Natural Science Foundation of Beijing, China (7002030). The nucleotide sequence reported in this paper has been submitted to the GenBank/EMBL/DDBJ nucleotide sequence databases under the accession No. AF119916.3 for human netrin-4_v2 cDNA.
PY - 2004/11/4
Y1 - 2004/11/4
N2 - Netrins are a family of secreted proteins that function as tropic cues directing axon growth and cell migration during neural development. Beta-netrin or netrin-4 (NTN4), the fourth member of this family, was previously reported by Koch (M. Koch, J.R. Murrell, D.D. Hunter, P.F. Olson, W. Jin, D.R. Keene, W.J. Brunken, R.E. Burgeson. A novel member of the netrin family, beta-netrin, shares homology with the beta chain of laminin: identification, expression, and functional characterization. J. Cell Biol. 151 (2000) 221-234) and Yin (Y. Yin, J.R. Sanes, J.H. Miner, Identification and expression of mouse netrin-4. Mech. Dev. 96 (2000) 115-119), respectively. However, whether there is another isoform of netrin-4 and the expression patterns have not been fully determined. Here we report the cloning of a novel isoform of human netrin-4 using rapid amplification of complementary DNA (cDNA) ends (RACE) technique and analysis on the netrin-4 mRNA expression in adult rat brain using in situ hybridization. The new isoform lacks the signal peptide region and might represent a truncated form at the Laminin-N terminal domain of human netrin-4. Examination of the expression pattern of netrin-4 mRNA in adult rat brain revealed that the netrin-4 mRNA was widely expressed in many regions of the brain. High levels of netrin-4 mRNA was found in the pyramidal cell layer of the cerebral cortex, prepiriform cortex, amygdaloid nuclei, pyramidal layer of hippocampus, Purkinje's cells, medial cerebellar nucleus and interposed cerebellar nucleus, medial nucleus of the trapezoid body and mitral cell layer of the olfactory bulb. Moderate signals were present in frontal cortex, several thalamic and hypothalamic nuclei. The widespread expression of netrin-4 in adult rat brain indicates that netrin-4 is not only important for axon guidance during embryonic development, but also critical to neuronal plasticity in adult stage.
AB - Netrins are a family of secreted proteins that function as tropic cues directing axon growth and cell migration during neural development. Beta-netrin or netrin-4 (NTN4), the fourth member of this family, was previously reported by Koch (M. Koch, J.R. Murrell, D.D. Hunter, P.F. Olson, W. Jin, D.R. Keene, W.J. Brunken, R.E. Burgeson. A novel member of the netrin family, beta-netrin, shares homology with the beta chain of laminin: identification, expression, and functional characterization. J. Cell Biol. 151 (2000) 221-234) and Yin (Y. Yin, J.R. Sanes, J.H. Miner, Identification and expression of mouse netrin-4. Mech. Dev. 96 (2000) 115-119), respectively. However, whether there is another isoform of netrin-4 and the expression patterns have not been fully determined. Here we report the cloning of a novel isoform of human netrin-4 using rapid amplification of complementary DNA (cDNA) ends (RACE) technique and analysis on the netrin-4 mRNA expression in adult rat brain using in situ hybridization. The new isoform lacks the signal peptide region and might represent a truncated form at the Laminin-N terminal domain of human netrin-4. Examination of the expression pattern of netrin-4 mRNA in adult rat brain revealed that the netrin-4 mRNA was widely expressed in many regions of the brain. High levels of netrin-4 mRNA was found in the pyramidal cell layer of the cerebral cortex, prepiriform cortex, amygdaloid nuclei, pyramidal layer of hippocampus, Purkinje's cells, medial cerebellar nucleus and interposed cerebellar nucleus, medial nucleus of the trapezoid body and mitral cell layer of the olfactory bulb. Moderate signals were present in frontal cortex, several thalamic and hypothalamic nuclei. The widespread expression of netrin-4 in adult rat brain indicates that netrin-4 is not only important for axon guidance during embryonic development, but also critical to neuronal plasticity in adult stage.
KW - Adult rat
KW - Alternative splicing
KW - Brain
KW - In situ hybridization
KW - Netrin
KW - Netrin-4
KW - Rapid amplification of cDNA ends
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U2 - 10.1016/j.molbrainres.2004.07.009
DO - 10.1016/j.molbrainres.2004.07.009
M3 - Article
C2 - 15519678
AN - SCOPUS:7444231094
SN - 0169-328X
VL - 130
SP - 68
EP - 80
JO - Molecular Brain Research
JF - Molecular Brain Research
IS - 1-2
ER -