Identification of a previously unknown human collagen chain, α1(XV), characterized by extensive interruptions in the triple-helical region

Jeanne C. Myers, Sirpa Kivirikko, Marion Gordon, Arnold S. Dion, Taina Pihlajaniemi

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

A previously unknown collagen cDNA clone, PF19, was isolated from a human placenta library. The 2.1-kilobase insert has a complete open reading frame of 709 amino acids that includes 12 amino acids of the NH2-terminal domain, a principally collagenous region of 577 residues, and 120 residues of the noncollagenous COOH terminus. The collagenous part of the sequence encoded by PF19 is characterized by 13 interruptions ranging in size from 2 to 45 amino acids. Within four interruptions are consensus sequences for attachment of serine-linked glycosaminoglycans and asparagine-linked oligosaccharides suggesting that this collagen may be extensively glycosylated. A synthetic decapeptide representing a sequence at the beginning of the COOH-terminal noncollagenous domain was used to prepare an antibody in rabbits. This antiserum detected a 125-kDa bacterial collagenase-sensitive protein in Western blots of HeLa cell lysate. Consistent with the size of the collagen chain, Northern blot hybridization revealed a major transcript of 5.3 kilobases and two minor ones of 4.7 and 4.4 kilobases that are present in cultured human fibroblasts but absent from umbilical vein endothelial cells. We propose that the previously unidentified polypeptide described in this report be designated the al chain of type XV collagen.

Original languageEnglish (US)
Pages (from-to)10144-10148
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number21
DOIs
StatePublished - Jan 1 1992

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Collagen
Amino Acids
Umbilical Veins
Asparagine
Consensus Sequence
Collagenases
Glycosaminoglycans
Oligosaccharides
HeLa Cells
Northern Blotting
Serine
Placenta
Open Reading Frames
Immune Sera
Endothelial Cells
Complementary DNA
Clone Cells
Fibroblasts
Western Blotting
Rabbits

All Science Journal Classification (ASJC) codes

  • General

Keywords

  • Extracellular matrix
  • Glycosylation
  • Multiple RNAs
  • Type XV collagen
  • cDNA clones

Cite this

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title = "Identification of a previously unknown human collagen chain, α1(XV), characterized by extensive interruptions in the triple-helical region",
abstract = "A previously unknown collagen cDNA clone, PF19, was isolated from a human placenta library. The 2.1-kilobase insert has a complete open reading frame of 709 amino acids that includes 12 amino acids of the NH2-terminal domain, a principally collagenous region of 577 residues, and 120 residues of the noncollagenous COOH terminus. The collagenous part of the sequence encoded by PF19 is characterized by 13 interruptions ranging in size from 2 to 45 amino acids. Within four interruptions are consensus sequences for attachment of serine-linked glycosaminoglycans and asparagine-linked oligosaccharides suggesting that this collagen may be extensively glycosylated. A synthetic decapeptide representing a sequence at the beginning of the COOH-terminal noncollagenous domain was used to prepare an antibody in rabbits. This antiserum detected a 125-kDa bacterial collagenase-sensitive protein in Western blots of HeLa cell lysate. Consistent with the size of the collagen chain, Northern blot hybridization revealed a major transcript of 5.3 kilobases and two minor ones of 4.7 and 4.4 kilobases that are present in cultured human fibroblasts but absent from umbilical vein endothelial cells. We propose that the previously unidentified polypeptide described in this report be designated the al chain of type XV collagen.",
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Identification of a previously unknown human collagen chain, α1(XV), characterized by extensive interruptions in the triple-helical region. / Myers, Jeanne C.; Kivirikko, Sirpa; Gordon, Marion; Dion, Arnold S.; Pihlajaniemi, Taina.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, No. 21, 01.01.1992, p. 10144-10148.

Research output: Contribution to journalArticle

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T1 - Identification of a previously unknown human collagen chain, α1(XV), characterized by extensive interruptions in the triple-helical region

AU - Myers, Jeanne C.

AU - Kivirikko, Sirpa

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AU - Pihlajaniemi, Taina

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