Identification of a receptor for protein import into mitochondria

Debkumar Pain, Hiroshi Murakami, Günter Blobel

Research output: Contribution to journalArticlepeer-review

84 Scopus citations


Anti-idiotypic antibodies, prepared using a chemically synthesized signal peptide of a mitochondrial precursor protein, recognized a mitochondrial integral membrane protein (p32). Fab fragments derived from both anti-idiotypic antibodies and monospecific antibodies against purified p32 inhibited protein import into mitochondria. Moreover, anti-p32 antibodies specifically immunoprecipitated a precursor-p32 complex after detergent solubilization of mitochondria. Immunoelectron microscopy and subfractionation of mitochondria indicate that p32 is located in contact sites between the outer and inner mitochondrial membranes.

Original languageEnglish (US)
Pages (from-to)444-449
Number of pages6
Issue number6292
StatePublished - 1990
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General


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