Identification of cDNA clones for ligninase from Phanerochaete chrysosporium using synthetic oligonucleotide probes

Y. Z. Zhang, Gerben Zylstra, R. H. Olsen, C. A. Reddy

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Four cDNA clones for ligninase were isolated from the cDNA library (constructed into the PstI site of E. coli vector pUC9) representing 6 day-old lignin degrading culture of Phanerochaete chrysosporium by the use of three synthetic oligonucleotide probes corresponding to partial amino acid sequences of tryptic peptides of the ligninase. Each of the three probes, 14.1, 14.2 and 25, represents a mixture of 32 12- or 14-base long oligonucleotides. Three cDNA clones hybridized with probe 14.1 but not with probe 25 or 14.2, but one cDNA clone hybridized with all of the three probes. Differential hybridization studies showed that these clones are unique to 6-day poly(A) RNA, but not to 2-day poly(A) RNA.

Original languageEnglish (US)
Pages (from-to)649-656
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume137
Issue number2
DOIs
StatePublished - Jun 13 1986
Externally publishedYes

Fingerprint

Phanerochaete
Oligonucleotide Probes
Complementary DNA
Clone Cells
Messenger RNA
Lignin
Gene Library
Oligonucleotides
Escherichia coli
Amino Acid Sequence
Amino Acids
Peptides
ligninase

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

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abstract = "Four cDNA clones for ligninase were isolated from the cDNA library (constructed into the PstI site of E. coli vector pUC9) representing 6 day-old lignin degrading culture of Phanerochaete chrysosporium by the use of three synthetic oligonucleotide probes corresponding to partial amino acid sequences of tryptic peptides of the ligninase. Each of the three probes, 14.1, 14.2 and 25, represents a mixture of 32 12- or 14-base long oligonucleotides. Three cDNA clones hybridized with probe 14.1 but not with probe 25 or 14.2, but one cDNA clone hybridized with all of the three probes. Differential hybridization studies showed that these clones are unique to 6-day poly(A) RNA, but not to 2-day poly(A) RNA.",
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Identification of cDNA clones for ligninase from Phanerochaete chrysosporium using synthetic oligonucleotide probes. / Zhang, Y. Z.; Zylstra, Gerben; Olsen, R. H.; Reddy, C. A.

In: Biochemical and Biophysical Research Communications, Vol. 137, No. 2, 13.06.1986, p. 649-656.

Research output: Contribution to journalArticle

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AU - Zhang, Y. Z.

AU - Zylstra, Gerben

AU - Olsen, R. H.

AU - Reddy, C. A.

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