Identification of cDNAs corresponding to mosquito ribosomal protein genes

Joan E. Durbin, Mavis R. Swerdel, Ann Marie Fallon

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Sequences encoding mosquito (Aedes albopictus) ribosomal proteins L8, L14 and L31 were identified from a cDNA library made from size-selected polyadenylated mRNA. Candidate cDNAs corresponding to moderately abundant mRNAs were screened by translation of hybrid-selected transcripts in wheat-germ lysates. Translation products were extracted with acetic acid and analyzed by electrophoresis in two dimensions in the presence of unlabeled ribosomal proteins. The identity of translation products that coelectrophoresed with purified ribosomal protein standards was supported by peptide mapping. The cDNAs corresponding to L8 (pL8) and L31 (pL31) hybridized to cytoplasmic mRNAs of 1.4 and 0.9 kb, respectively. In Southern blots of genomic DNA digested with BamHI, HindIII or EcoRI, the cDNA inserts from both pL8 and pL31 gave simple hybridization patterns suggestive of a low copy number for mosquito ribosomal protein genes.

Original languageEnglish (US)
Pages (from-to)182-192
Number of pages11
JournalBBA - Gene Structure and Expression
Volume950
Issue number2
DOIs
StatePublished - Jul 13 1988

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Biophysics
  • Biochemistry
  • Genetics

Keywords

  • (A. albopictus)
  • (Mosquito)
  • Ribosomal protein gene
  • cDNA

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