Identification of critical members in a sulfidogenic benzene-degrading consortium by DNA stable isotope probing

A. R. Oka; C. D. Phelps; L. M. McGuinness; A. Mumford; L. Y. Young; L. J. Kerkhof

doi:10.1128/AEM.01082-08

Identification of critical members in a sulfidogenic benzene-degrading consortium by DNA stable isotope probing

A. R. Oka, C. D. Phelps, L. M. McGuinness, A. Mumford, L. Y. Young, L. J. Kerkhof

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Abstract

Stable isotope probing (SIP) was used to identify the active members in a benzene-degrading sulfidogenic consortium. SIP-terminal restriction fragment length polymorphism analysis indicated that a 270-bp peak incorporated the majority of the ¹³C label and is a sequence closely related to that of clone SB-21 (GenBank accession no. AF029045). This target may be an important biomarker for anaerobic benzene degradation in the field.

Original language	English (US)
Pages (from-to)	6476-6480
Number of pages	5
Journal	Applied and environmental microbiology
Volume	74
Issue number	20
DOIs	https://doi.org/10.1128/AEM.01082-08
State	Published - Oct 2008

All Science Journal Classification (ASJC) codes

Biotechnology
Food Science
Ecology
Applied Microbiology and Biotechnology

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abstract = "Stable isotope probing (SIP) was used to identify the active members in a benzene-degrading sulfidogenic consortium. SIP-terminal restriction fragment length polymorphism analysis indicated that a 270-bp peak incorporated the majority of the 13C label and is a sequence closely related to that of clone SB-21 (GenBank accession no. AF029045). This target may be an important biomarker for anaerobic benzene degradation in the field.",

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AU - Oka, A. R.

AU - Phelps, C. D.

AU - McGuinness, L. M.

AU - Mumford, A.

AU - Young, L. Y.

AU - Kerkhof, L. J.

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AB - Stable isotope probing (SIP) was used to identify the active members in a benzene-degrading sulfidogenic consortium. SIP-terminal restriction fragment length polymorphism analysis indicated that a 270-bp peak incorporated the majority of the 13C label and is a sequence closely related to that of clone SB-21 (GenBank accession no. AF029045). This target may be an important biomarker for anaerobic benzene degradation in the field.

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Identification of critical members in a sulfidogenic benzene-degrading consortium by DNA stable isotope probing

Abstract

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