Identification of cytokeratins as accessory mediators of Salmonella entry into eukaryotic cells

Steve A. Carlson, M. Bishr Omary, Bradley D. Jones

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

Pathogenic Salmonella species initiate infection of a mammalian host by inducing their own uptake into intestinal M-cells. During the uptake process, the bacteria utilize an intrinsic secretion system to release proteins that enter host cells. The secreted invasion-mediating proteins subsequently interact with host cell components that induce alterations in the actin cytoskeleton. To identify potential cellular determinants of invasion, we employed a yeast two-hybrid system using the secreted Salmonella invasion protein (SipC) as the bait protein. This system identified cytokeratins, supportive components of the cytoskeletal matrix, as proteins that may physically interact with SipC. Transfection-based studies revealed an inhibition of Salmonella invasion when a dominant negative cytokeratin-18 was expressed. Immunofluorescent confocal microscopy studies revealed that Salmonella did not enter HEp-2 cells expressing the dominant negative cytokeratin-18. These results suggest that an interaction between SipC and cytokeratin-18 may occur as part of Salmonella invasion.

Original languageEnglish (US)
Pages (from-to)1415-1426
Number of pages12
JournalLife Sciences
Volume70
Issue number12
DOIs
StatePublished - Feb 8 2002
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General Pharmacology, Toxicology and Pharmaceutics
  • General Biochemistry, Genetics and Molecular Biology

Keywords

  • Cytokeratins
  • Invasion
  • Salmonella
  • SipC protein
  • Virulence

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