Microcosms utilizing groundwater and sediment fines from a monitoring well in a tetrachloroethylene (PCE)-contaminated fractured rock aquifer at Rutgers University were developed. Five sets of microcosms were established (killed controls, live controls, electron donors only, electron donors + PCE, and electron donors + vinyl chloride (VC)). Complete dechlorination to ethylene occurred in the original, electron donors + PCE and VC amended microcosms. The microbial community was characterized using genus-specific PCR primers and denaturing gradient gel electrophoresis. Dehalococcoides-like bacteria were detected using nested and direct PCR with a Dehalococcoides specific primer set. This is an abstract of a paper presented at the 228th ACS National Meeting (Philadelphia, PA 8/22-26/2004).
All Science Journal Classification (ASJC) codes
- Chemical Engineering(all)