Identification of epidermal growth factor receptor in human buccal mucosa

S. L. Wang, M. Milles, C. Y. Wu-Wang, J. Liu, Amalia Slomiany, Bronislaw Slomiany

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

EGF receptor was identified and its binding characteristics were determined. Buccal mucosa was obtained from 12 healthy volunteers (6 males and 6 females) and assayed individually for [125I]-EGF binding. The specific binding of [125I]-EGF to the receptor ranged from 2.85 to 6.12 fmol/mg protein. There was no significant difference in binding between male and female (4.31 ± 0.61 versus 3.94 ± 0.53 fmol/mg protein; mean ± SEM). Individual tissue homogenates were pooled for Scatchard analysis and cross-linking experiments. Scatchard analysis produced curvilinear plots with a Kd of 0.71 nM and Bmax of 0.024 pmol/mg protein for the high-affinity binding sites, and Kd of 435 nM and Bmax of 9.92 pmol/mg protein for the low-affinity binding sites. To determine the molecular weight of the EGF receptor, the [125I]-EGF and receptor complex were cross-linked by DSS and subjected to SDS-PAGE. The autoradiogram of the gel revealed one major protein band of 160 K and a minor band of 170 K, characteristics shared with the EGF receptors in other tissues. The study is thought to be the first to demonstrate the presence of the EGF receptor in human buccal tissue and to show its biochemical features.

Original languageEnglish (US)
Pages (from-to)823-828
Number of pages6
JournalArchives of Oral Biology
Volume35
Issue number10
DOIs
StatePublished - Jan 1 1990

Fingerprint

Mouth Mucosa
Epidermal Growth Factor Receptor
Proteins
Binding Sites
Cheek
Epidermal Growth Factor
Polyacrylamide Gel Electrophoresis
Healthy Volunteers
Molecular Weight
Gels

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology
  • Dentistry(all)
  • Cell Biology

Keywords

  • buccal mucosa
  • epidermal growth factor
  • human
  • receptor

Cite this

Wang, S. L. ; Milles, M. ; Wu-Wang, C. Y. ; Liu, J. ; Slomiany, Amalia ; Slomiany, Bronislaw. / Identification of epidermal growth factor receptor in human buccal mucosa. In: Archives of Oral Biology. 1990 ; Vol. 35, No. 10. pp. 823-828.
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Identification of epidermal growth factor receptor in human buccal mucosa. / Wang, S. L.; Milles, M.; Wu-Wang, C. Y.; Liu, J.; Slomiany, Amalia; Slomiany, Bronislaw.

In: Archives of Oral Biology, Vol. 35, No. 10, 01.01.1990, p. 823-828.

Research output: Contribution to journalArticle

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