Identification of functional cAMP-dependent protein kinase in a 'neurite minus' mouse neuroblastoma cell line (BBA 10201)

Alice Liu, V. Kamalakannan, Kuang Yu Chen

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

We have characterized and quantitated the level of cAMP-dependent protein kinase in the NS-20, N1E-115, N-18 and N1A-103 mouse neuroblastoma clonal cell lines, and we have correlated the occurrence of functional cAMP-dependent protein kinase with the dibutyryl cAMP-induced differentiated functions in these cells. Our results demonstrate the presence of functional cAMP-dependent protein kinase in extracts of all four cell lines examined, including the 'neurite minus' N1A-103 cell line. Dibutyryl cAMP induced neurite outgrowth and acetylcholinesterase activity in the NS-20, N1E-115 and N-18 neuroblastoma cell lines, but not in the N1A-103 cell line. However, dibutyryl cAMP caused a 2-3-fold increase in the R1 regulatory subunit protein and cAMP-phosphodiesterase activity in the 'neurite minus' N1A-103 cells in a manner similar to that of the other three 'neurite positive' cell lines. These results suggest that the biochemical lesion(s) subserving the neurite-minus phenotype of the N1A-103 cells may be distal to the activation of cAMP-dependent protein kinase and is in a biochemical pathway distinct from the induction of RI regulatory subunit protein and cAMP-phosphodiesterase activity.

Original languageEnglish (US)
Pages (from-to)235-239
Number of pages5
JournalBBA - Molecular Cell Research
Volume928
Issue number2
DOIs
StatePublished - Apr 22 1987

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Keywords

  • Differentiation
  • Neuroblastoma
  • Protein kinase, cAMP-dependent

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