Identification of protein bound to the termini of bacteriophage PRD1 DNA

D. Bamford, T. McGraw, G. MacKenzie, L. Mindich

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

Lipid-containing bacteriophage PRD1 has a double-stranded DNA genome of about 14,500 nucleotide base pairs. The phage can infect Escherichia coli and Salmonella typhimurium as well as other gram-negative bacteria harboring an appropriate plasmid. [35S]methionine label is incorporated into the DNA band early in infection. The label remains associated with DNA through phenol extraction and boiling with sodium dodecyl sulfate. Nuclease treatment of the genome released a protein which migrated as an early phage-specific protein (P8). This protein is also necessary for phage DNA replication. By restriction enzyme analysis it was shown that protein was associated with the terminal restriction fragments. Extracts of infected cells catalyzed the labeling of protein P8 with [α-32P]dGTP.

Original languageEnglish (US)
Pages (from-to)311-316
Number of pages6
JournalJournal of virology
Volume47
Issue number2
DOIs
StatePublished - 1983

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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