Identification of sanguinarine as a novel HIV protease inhibitor from high-throughput screening of 2,000 drugs and natural products with a cell-based assay

Ting Jen Cheng; David S. Goodsell; Chen Chen Kan

doi:10.2174/1570180054405811

Identification of sanguinarine as a novel HIV protease inhibitor from high-throughput screening of 2,000 drugs and natural products with a cell-based assay

Ting Jen Cheng, David S. Goodsell, Chen Chen Kan

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

We identified a non-peptidomimetic HIV protease inhibitor sanguinarine through screening a small compound library with a high-throughput E. coli-based assay. In vitro, sanguinarine inhibits the HIV protease with an IC₅₀ of around 13 μM. Based on docking results, binding modes of sanguinarine to the HIV protease are proposed.

Original language	English (US)
Pages (from-to)	364-371
Number of pages	8
Journal	Letters in Drug Design and Discovery
Volume	2
Issue number	5
DOIs	https://doi.org/10.2174/1570180054405811
State	Published - Aug 2005
Externally published	Yes

All Science Journal Classification (ASJC) codes

Molecular Medicine
Pharmaceutical Science
Drug Discovery

Keywords

E. coli-based system
HIV protease
HIV protease inhibitors
HIV protease precursor
High-throughput screening
Sanguinarine

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10.2174/1570180054405811

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title = "Identification of sanguinarine as a novel HIV protease inhibitor from high-throughput screening of 2,000 drugs and natural products with a cell-based assay",

abstract = "We identified a non-peptidomimetic HIV protease inhibitor sanguinarine through screening a small compound library with a high-throughput E. coli-based assay. In vitro, sanguinarine inhibits the HIV protease with an IC50 of around 13 μM. Based on docking results, binding modes of sanguinarine to the HIV protease are proposed.",

keywords = "E. coli-based system, HIV protease, HIV protease inhibitors, HIV protease precursor, High-throughput screening, Sanguinarine",

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AU - Goodsell, David S.

AU - Kan, Chen Chen

PY - 2005/8

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AB - We identified a non-peptidomimetic HIV protease inhibitor sanguinarine through screening a small compound library with a high-throughput E. coli-based assay. In vitro, sanguinarine inhibits the HIV protease with an IC50 of around 13 μM. Based on docking results, binding modes of sanguinarine to the HIV protease are proposed.

KW - E. coli-based system

KW - HIV protease

KW - HIV protease inhibitors

KW - HIV protease precursor

KW - High-throughput screening

KW - Sanguinarine

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Identification of sanguinarine as a novel HIV protease inhibitor from high-throughput screening of 2,000 drugs and natural products with a cell-based assay

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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